Department of Biomedical Engineering, Washington University, St. Louis, MO 63130, USA.
Angew Chem Int Ed Engl. 2010 Mar 8;49(11):1976-80. doi: 10.1002/anie.200906584.
This paper presents a simple spectroscopic method for differentiating cellular uptakes of two different types of Au nanostructures (i.e., nanospheres and nanorods) when they are supplied as a mixture. Because Au nanospheres and nanorods have distinctive surface plasmon resonance peaks, we can simultaneously measure their concentrations in a culture mediumbyUV-Vis spectroscopy. For the uptake of Au nanostructures by SK-BR-3 breast cancer cells, we found that the surface functional group had a stronger influence than the shape. We also found that the cellular uptake of one type of Au nanostructure could be affected bythe presence of another type of nanostructure in the medium. This study not only suggests a simple way to compare the cellular uptakes of more than one type of Au nanostructures without incubating them separately but also allows one to investigate the influence of one type of Au nanostructures on the uptake of another type.
本文提出了一种简单的光谱方法，可用于区分当两种不同类型的金纳米结构（即纳米球和纳米棒）作为混合物供应时的细胞摄取。由于金纳米球和纳米棒具有独特的表面等离子体共振峰，我们可以通过紫外-可见光谱同时测量它们在培养基中的浓度。对于 SK-BR-3 乳腺癌细胞对金纳米结构的摄取，我们发现表面官能团的影响比形状更强。我们还发现，一种金纳米结构的细胞摄取可以被介质中另一种纳米结构的存在所影响。这项研究不仅提出了一种简单的方法来比较一种以上类型的金纳米结构的细胞摄取，而无需分别孵育它们，还允许研究一种金纳米结构对另一种纳米结构摄取的影响。
