Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, P.O. Box 1417614411, Iran.
Department of Electrical Engineering, Amirkabir University of Technology, Tehran, P.O.Box 1591634311, Iran.
Colloids Surf B Biointerfaces. 2019 Jan 1;173:891-898. doi: 10.1016/j.colsurfb.2018.10.042. Epub 2018 Oct 17.
Interaction of nanoplatforms with biomolecules in biological fluids alters nanoplatforms approach to target tissue and deliver their cargo. Here in, three nanoplatforms were utilized as a carrier to detect the effects of subsequent biomolecules on gene delivery using NIR thermal therapy. Nanoplatforms included; graphene oxide coated gold nanorods (NR@GO), PEGylated NR@GO (NR@GO-PEG) and poly L arginine functionalized NR@GO-PEG (NR@GO-PEG-PLArg). Results indicated that incubation of nanoplatforms in different concentrations of human plasma induced the evolution of layer of proteins (corona) with different thickness on the surface of nanoplatforms. Protein corona decreased the surface charge and optical properties of nanoplatforms. Corona subunits of ITIH, HAS and APOs protein family were extracted from NR@GO-PEG-PLArg surface that play a major role in cellular internalization of nanoplatforms. Moreover, NR@GO-PEG-PLArg remarkably targeted the cancer cells due to uncovered long linear chains of targeting agent (PLArg). The process of gene release and activating apoptotic pathway were enhanced by NIR thermal therapy, which could disrupt the electrostatic interactions and release the protein corona and genes from the surface of nanoplatforms. In conclusion, modification of nanoplatforms with targeting agents could alter the composition of corona toward well interaction with cell and deliver the therapeutic agent.
纳米平台与生物体液中的生物分子相互作用会改变纳米平台接近靶组织的方式，并输送其货物。在这里，使用近红外热疗研究了三种纳米平台作为载体检测随后的生物分子对基因传递的影响。纳米平台包括：氧化石墨烯包覆的金纳米棒（NR@GO）、聚乙二醇化 NR@GO（NR@GO-PEG）和聚 L 精氨酸功能化 NR@GO-PEG（NR@GO-PEG-PLArg）。结果表明，纳米平台在不同浓度的人血浆中孵育会导致纳米平台表面形成不同厚度的蛋白质层（蛋白冠）。蛋白冠降低了纳米平台的表面电荷和光学性质。从 NR@GO-PEG-PLArg 表面提取出 ITIH、HAS 和 APOs 蛋白家族的蛋白冠亚基，这些亚基在纳米平台的细胞内化中起主要作用。此外，由于靶向剂（PLArg）的未覆盖的长线性链，NR@GO-PEG-PLArg 显著靶向癌细胞。近红外热疗增强了基因释放和激活凋亡途径的过程，这可以破坏静电相互作用并从纳米平台表面释放蛋白冠和基因。总之，用靶向剂修饰纳米平台可以改变蛋白冠的组成，以更好地与细胞相互作用并输送治疗剂。
