LEF-EGFP Wnt报告基因在小鼠发育和癌症中的动态表达。
Dynamic expression of a LEF-EGFP Wnt reporter in mouse development and cancer.
作者信息
Currier Nicolas, Chea Kathleen, Hlavacova Mirka, Sussman Daniel J, Seldin David C, Dominguez Isabel
机构信息
Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, USA.
出版信息
Genesis. 2010 Mar;48(3):183-94. doi: 10.1002/dvg.20604.
Abstract
We have characterized a transgenic mouse line in which enhanced green fluorescent protein (EGFP) is expressed under the control of multimerized LEF-1 responsive elements. In embryos, EGFP was detected in known sites of Wnt activation, including the primitive streak, mesoderm, neural tube, somites, heart, limb buds, mammary placodes, and whisker follicles. In vitro cultured transgenic embryonic fibroblasts upregulated EGFP expression in response to activation of Wnt signaling by GSK3beta inhibition. Mammary tumor cell lines derived from female LEF-EGFP transgenic mice treated with the carcinogen 7, 12-dimethylbenz[a]anthracene (DMBA) also express EGFP. Thus, this transgenic line is useful for ex vivo and in vitro studies of Wnt signaling in development and cancer.
摘要
我们已经鉴定了一种转基因小鼠品系，其中增强型绿色荧光蛋白（EGFP）在多聚化的LEF-1反应元件的控制下表达。在胚胎中，在已知的Wnt激活位点检测到EGFP，包括原条、中胚层、神经管、体节、心脏、肢芽、乳腺基板和触须毛囊。体外培养的转基因胚胎成纤维细胞在受到GSK3β抑制激活Wnt信号时会上调EGFP表达。用致癌物7,12-二甲基苯并[a]蒽（DMBA）处理的雌性LEF-EGFP转基因小鼠衍生的乳腺肿瘤细胞系也表达EGFP。因此，该转基因品系可用于Wnt信号在发育和癌症中的体外和体内研究。
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