SBÜ Bağcılar Eğitim ve Araştırma Hastanesi Ortopedi ve Travmatoloji Kliniği, 34200 Bağcılar, İstanbul, Türkiye.
Jt Dis Relat Surg. 2022;33(3):553-566. doi: 10.52312/jdrs.2022.752. Epub 2022 Oct 21.
In this study, we aimed to evaluate the antioxidant, anti-apoptotic, osteoblastic and hypolipidemic effects of thymoquinone (TQ) treatment on the steroid-induced osteonecrosis of femoral head (ONFH) model in rats.
A total of 24 rats were randomly divided into four groups: the control group administered saline; the TQ group administered 10 mg/kg/day TQ orally; lipopolysaccharide/methylprednisolone (LPS/MPS) group administered 20 µg/kg intraperitoneally LPS and 40 mg/kg intramuscularly MPS to establish ONFH model; and the LPS/ MPS+TQ group administered both LPS/MPS and, then, TQ once daily for four weeks. All rats were sacrificed after intracardiac blood collection and their right femurs were removed.
Micro-computed tomography showed a higher bone mineral density and lower porosity, Tr. Sp and Tr. Sep data were detected in the LPS/MPS+TQ group. In histopathology, osteonecrosis increased significantly in the LPS/MPS group and osteonecrosis decreased in the LPS/MPS+TQ group compared to the LPS/MPS group (p=0.0077). Histomorphometric examination revealed that the percentage of BV/TV in the LPS/MPS group was significantly lower compared to control and other groups (p<0.01 and p<0.05, respectively), while it reached normal rates in the LPS/MPS+TQ group. Immunohistochemically, antioxidant, anti-apoptotic, and angiogenesis indicators (8-hydroxy-20- deoxyguanosine [8-OHdG], malondialdehyde [MDA], B-cell lymphoma [Bcl-2], caspase-3, vascular endothelial growth factor [VEGF]) were significantly improved in tissue and serum with TQ. Furthermore, TQ significantly reduced low- and high-density lipoprotein cholesterol ratio and carboxy-terminal type 1 collagen crosslink (CTX) in serum.
Vascular and hematopoietic cell damages that occur due to steroid-induced deoxyribonucleic acid (DNA) oxidative and lipid peroxidative damages in an ONFH model can be successfully ameliorated by TQ administration. This antioxidant and anti-apoptotic effects of TQ may be a promising treatment option for early stage of osteonecrosis.
本研究旨在评估胸腺醌（TQ）治疗对大鼠类固醇诱导性股骨头坏死（ONFH）模型的抗氧化、抗凋亡、成骨和降血脂作用。
将 24 只大鼠随机分为四组：对照组给予生理盐水；TQ 组给予 10mg/kg/天 TQ 口服；脂多糖/甲基强的松龙（LPS/MPS）组给予 20μg/kg 腹腔内 LPS 和 40mg/kg 肌肉内 MPS 建立 ONFH 模型；LPS/MPS+TQ 组给予 LPS/MPS 后，每天给予 TQ 一次，共四周。所有大鼠在心脏采血后处死，取出右侧股骨。
Micro-CT 显示骨矿物质密度较高，孔隙率较低，LPS/MPS+TQ 组 Tr.Sp 和 Tr.Sep 数据较高。组织病理学检查显示，LPS/MPS 组骨坏死明显增加，LPS/MPS+TQ 组骨坏死较 LPS/MPS 组减少（p=0.0077）。组织形态计量学检查显示，LPS/MPS 组的 BV/TV 百分比明显低于对照组和其他组（p<0.01 和 p<0.05），而 LPS/MPS+TQ 组恢复正常。免疫组织化学染色显示，TQ 可显著改善组织和血清中的抗氧化、抗凋亡和血管生成指标（8-羟基-20-脱氧鸟苷[8-OHdG]、丙二醛[MDA]、B 细胞淋巴瘤[Bcl-2]、半胱氨酸天冬氨酸蛋白酶-3、血管内皮生长因子[VEGF]）。此外，TQ 可显著降低血清中低、高密度脂蛋白胆固醇比值和羧基末端型 1 胶原交联（CTX）。
TQ 可改善类固醇诱导的脱氧核糖核酸（DNA）氧化和脂质过氧化损伤所致 ONFH 模型中的血管和造血细胞损伤。TQ 的抗氧化和抗凋亡作用可能是治疗早期骨坏死的一种有前途的方法。
