实时聚合酶链反应在布隆迪溃疡流行国家加纳的应用证实了环境中存在溃疡分枝杆菌。

Application of real-time PCR in Ghana, a Buruli ulcer-endemic country, confirms the presence of Mycobacterium ulcerans in the environment.

机构信息

Mycobacteriology Unit, Department of Microbiology, Institute of Tropical Medicine, Antwerp, Belgium.

出版信息

FEMS Microbiol Lett. 2010 Mar;304(2):191-4. doi: 10.1111/j.1574-6968.2010.01902.x. Epub 2010 Jan 18.

DOI:10.1111/j.1574-6968.2010.01902.x

PMID:20146745

Abstract

This study reports the first successful application of real-time PCR for the detection of Mycobacterium ulcerans, the causative agent of Buruli ulcer (BU), in Ghana, a BU-endemic country. Environmental samples and organs of small mammals were analyzed. The real-time PCR assays confirmed the presence of M. ulcerans in a water sample collected in a BU-endemic village in the Ashanti Region.

摘要

本研究报告了实时 PCR 检测导致非洲溃疡病（BU）的溃疡分枝杆菌（Mycobacterium ulcerans）在加纳（BU 流行国家）的首次成功应用。对环境样本和小型哺乳动物器官进行了分析。实时 PCR 检测证实了在阿散蒂地区的一个 BU 流行村庄采集的水样中存在溃疡分枝杆菌。