Bustamante Carlos, Chemla Yann R, Moffitt Jeffrey R
Cold Spring Harb Protoc. 2009 Oct;2009(10):pdb.ip74. doi: 10.1101/pdb.ip74.
Optical traps or "optical tweezers" have become an indispensable tool in understanding fundamental biological processes. Dual-trap optical tweezers with differential detection have the advantage that the instrument is decoupled from sources of environmental noise, thereby minimizing interference of signal and maximizing stability and spatial resolution. In this article, we describe methods of data detection and calibration for our dual-trap design.
光镊或“光学镊子”已成为理解基本生物学过程中不可或缺的工具。具有差分检测功能的双阱光镊具有这样的优势，即该仪器与环境噪声源解耦，从而使信号干扰最小化，并使稳定性和空间分辨率最大化。在本文中，我们描述了我们双阱设计的数据检测和校准方法。
