Garcês Helena, Sinha Neelima
Department of Plant Biology, University of California, Davis, CA 95616, USA.
Cold Spring Harb Protoc. 2009 Oct;2009(10):pdb.prot5303. doi: 10.1101/pdb.prot5303.
Kalanchoë daigremontiana can be stably transformed using the Agrobacterium tumefaciens-mediated T-DNA transfer method, as described here. Sterilized plant tissue is cocultivated with an A. tumefaciens suspension, transformants are selected and the shoots are grown in rooting medium and then in soil. Plant phenotypes can be examined approximately 3 mo after transfer of plants to soil.
如本文所述，可使用根癌农杆菌介导的T-DNA转移方法对落地生根进行稳定转化。将经过消毒的植物组织与根癌农杆菌悬浮液共培养，筛选出转化体，使芽在生根培养基中生长，然后移栽到土壤中。将植株移栽到土壤中约3个月后，可对植物表型进行检测。
