Garcês Helena, Sinha Neelima
Department of Plant Biology, University of California, Davis, CA 95616, USA.
Cold Spring Harb Protoc. 2009 Oct;2009(10):pdb.prot5305. doi: 10.1101/pdb.prot5305.
This protocol describes how to isolate total RNA from several tissues of Kalanchoë daigremontiana. Total RNA can be isolated by using the TRI-reagent method, scaled up for processing 2 g of tissue, or by using the protocol described here, which gives higher concentrations of high quality RNA. The resulting RNA can be used for various applications including generation of cDNA for reverse transcriptase-PCR (RT-PCR), Northern blots, or other purposes.
