从植物落地生根中提取DNA。

Extraction of DNA from the plant Kalanchoë daigremontiana.

作者信息

Garcês Helena, Sinha Neelima

机构信息

Department of Plant Biology, University of California, Davis, CA 95616, USA.

出版信息

Cold Spring Harb Protoc. 2009 Oct;2009(10):pdb.prot5304. doi: 10.1101/pdb.prot5304.

DOI:10.1101/pdb.prot5304

PMID:20147049

Abstract

This protocol describes how to isolate genomic DNA from leaves and stems of Kalanchoë daigremontiana. The procedure can be applied to adult leaves, but it is best to use younger leaves because they have fewer secondary metabolites and polysaccharides, which can interfere with the DNA extraction. The resulting DNA can be used for polymerase chain reactions (PCRs), Southern blots, or other applications.

摘要

本方案描述了如何从落地生根的叶片和茎中分离基因组DNA。该方法可应用于成年叶片，但最好使用较嫩的叶片，因为它们含有的次生代谢产物和多糖较少，这些物质会干扰DNA提取。所得到的DNA可用于聚合酶链反应（PCR）、Southern杂交或其他应用。