Werren John H, Loehlin David W
Department of Biology, University of Rochester, Rochester, NY 14627, USA.
Cold Spring Harb Protoc. 2009 Oct;2009(10):pdb.prot5313. doi: 10.1101/pdb.prot5313.
This protocol describes a standard method for collecting Nasonia wasps using "flyliver": liver remains fed upon by fly maggots (e.g., Sarcophaga) and collected after the mature larvae have dispersed. The flyliver, which contains a volatile substance that is very attractive to the wasps, is placed in a large (approximately 15-cm(2)) mesh bag hung in appropriate collection spots (e.g., near birds' nests or under culverts). Within the large mesh bag is a smaller mesh bag placed abutting the flyliver and containing four to six Sarcophaga pupae. These retain the wasps, which will enter the bag and begin stinging the hosts. The mesh bags can be made with standard nylon window screening, or any other material with mesh width large enough to permit entry of the wasps.
本方案描述了一种使用“蝇肝”收集丽蝇蛹集金小蜂的标准方法：蝇肝是指仍有蝇蛆（如麻蝇）取食的肝脏，在成熟幼虫分散后进行收集。蝇肝含有一种对黄蜂极具吸引力的挥发性物质，将其放置在挂于合适收集地点（如鸟巢附近或涵洞下方）的大（约15平方厘米）网袋中。在大网袋内，紧挨着蝇肝放置一个较小的网袋，其中装有四到六个麻蝇蛹。这些蛹能留住黄蜂，黄蜂会进入袋中并开始叮咬宿主。网袋可用标准的尼龙窗纱或任何其他网眼宽度足够大以允许黄蜂进入的材料制成。
