从异性恋人类免疫缺陷病毒 C 型传播对中获得的供体和受者环境需要高受体水平才能进入。
Donor and recipient envs from heterosexual human immunodeficiency virus subtype C transmission pairs require high receptor levels for entry.
机构信息
Emory Vaccine Center, Yerkes National Primate Research Center, Emory University, 954 Gatewood Rd., Atlanta, GA 30329, USA.
出版信息
J Virol. 2010 Apr;84(8):4100-4. doi: 10.1128/JVI.02068-09. Epub 2010 Feb 10.
Abstract
Compact, glycan-restricted envelope (Env) glycoproteins are selected during heterosexual transmission of subtype C HIV-1. Donor and recipient glycoproteins (Envs) from six transmission pairs were evaluated for entry into HeLa cells expressing different levels of CD4 and CCR5. Donor and recipient Envs demonstrated efficient entry into cells expressing high levels of CD4 and CCR5, and entry declined as CCR5 levels decreased. Infectivity for all Envs was severely impaired in cells expressing low levels of CD4, even at the highest CCR5 levels. In 5/6 pairs, there was no significant difference in efficiency of receptor utilization between the donor and recipient Envs in these HeLa-derived cell lines. Thus, HIV-1 transmission does not appear to select for viruses that can preferentially utilize low levels of entry receptors.
摘要
在 HIV-1 亚型 C 的异性传播过程中，会选择紧凑的聚糖受限包膜 (Env) 糖蛋白。评估了来自 6 对传播对的供体和受体糖蛋白 (Env)，以评估它们进入表达不同水平 CD4 和 CCR5 的 HeLa 细胞的能力。供体和受体 Env 均能有效进入表达高水平 CD4 和 CCR5 的细胞，而随着 CCR5 水平的降低，进入能力下降。即使在最高 CCR5 水平下，所有 Env 在表达低水平 CD4 的细胞中的感染性都严重受损。在 5/6 对中，在这些 HeLa 衍生的细胞系中，供体和受体 Env 利用受体的效率没有显著差异。因此，HIV-1 传播似乎不会选择能够优先利用低水平进入受体的病毒。
相似文献
1
Donor and recipient envs from heterosexual human immunodeficiency virus subtype C transmission pairs require high receptor levels for entry.从异性恋人类免疫缺陷病毒 C 型传播对中获得的供体和受者环境需要高受体水平才能进入。
J Virol. 2010 Apr;84(8):4100-4. doi: 10.1128/JVI.02068-09. Epub 2010 Feb 10.
2
Heterosexual transmission of human immunodeficiency virus type 1 subtype C: Macrophage tropism, alternative coreceptor use, and the molecular anatomy of CCR5 utilization.人类免疫缺陷病毒1型C亚型的异性传播：巨噬细胞嗜性、替代共受体使用及CCR5利用的分子剖析
J Virol. 2009 Aug;83(16):8208-20. doi: 10.1128/JVI.00296-09. Epub 2009 Jun 10.
3
Transmitted/founder and chronic HIV-1 envelope proteins are distinguished by differential utilization of CCR5.传播/创始型和慢性 HIV-1 包膜蛋白通过 CCR5 的不同利用来区分。
J Virol. 2013 Mar;87(5):2401-11. doi: 10.1128/JVI.02964-12. Epub 2012 Dec 26.
4
Distinct HIV-1 entry phenotypes are associated with transmission, subtype specificity, and resistance to broadly neutralizing antibodies.不同的HIV-1进入表型与传播、亚型特异性以及对广泛中和抗体的抗性相关。
Retrovirology. 2014 Jun 23;11:48. doi: 10.1186/1742-4690-11-48.
5
Linkages between HIV-1 specificity for CCR5 or CXCR4 and in vitro usage of alternative coreceptors during progressive HIV-1 subtype C infection.在 HIV-1 亚型 C 感染的进展过程中，HIV-1 对 CCR5 或 CXCR4 的特异性与体外使用替代核心受体之间的关联。
Retrovirology. 2013 Sep 16;10:98. doi: 10.1186/1742-4690-10-98.
6
HIV-1 clade C envelopes obtained from late stage symptomatic Indian patients varied in their ability towards relative CD4 usages and sensitivity to CCR5 antagonist TAK-779.从晚期症状性印度患者中获得的 HIV-1 分群 C 包膜在相对 CD4 使用能力和对 CCR5 拮抗剂 TAK-779 的敏感性方面存在差异。
Virus Res. 2011 Jun;158(1-2):216-24. doi: 10.1016/j.virusres.2011.04.003. Epub 2011 Apr 15.
7
Longitudinal analysis of subtype C envelope tropism for memory CD4 T cell subsets over the first 3 years of untreated HIV-1 infection.在未经治疗的 HIV-1 感染的头 3 年内，对记忆性 CD4 T 细胞亚群中 C 型包膜嗜性的纵向分析。
Retrovirology. 2020 Aug 6;17(1):24. doi: 10.1186/s12977-020-00532-2.
8
HIV-1 non-macrophage-tropic R5 envelope glycoproteins are not more tropic for entry into primary CD4+ T-cells than envelopes highly adapted for macrophages.与高度适应巨噬细胞的包膜相比，HIV-1非巨噬细胞嗜性R5包膜糖蛋白对进入原代CD4+T细胞的嗜性并不更强。
Retrovirology. 2015 Mar 14;12:25. doi: 10.1186/s12977-015-0141-0.
9
Constrained use of CCR5 on CD4+ lymphocytes by R5X4 HIV-1: efficiency of Env-CCR5 interactions and low CCR5 expression determine a range of restricted CCR5-mediated entry.R5X4 HIV-1 对 CD4+ 淋巴细胞上 CCR5 的受限使用：Env-CCR5 相互作用的效率和低 CCR5 表达决定了受限的 CCR5 介导进入的范围。
Virology. 2010 Jun 20;402(1):135-48. doi: 10.1016/j.virol.2010.03.009. Epub 2010 Apr 9.
10
C-C chemokine receptor type 5 (CCR5) utilization of transmitted and early founder human immunodeficiency virus type 1 envelopes and sensitivity to small-molecule CCR5 inhibitors.C-C 趋化因子受体 5（CCR5）对传播和早期创始人类免疫缺陷病毒 1 包膜的利用以及对小分子 CCR5 抑制剂的敏感性。
J Gen Virol. 2010 Dec;91(Pt 12):2965-73. doi: 10.1099/vir.0.025270-0. Epub 2010 Sep 1.
引用本文的文献
1
Transmitted/founder (T/F) HIV-1 derived from sexual contact exhibits greater transmission fitness in human cervical tissue than T/F HIV-1 from blood-to-blood contact: Unique glycan profiles on T/F envelopes associated with transmission phenotypes.通过性接触传播的/奠基者（T/F）HIV-1在人宫颈组织中的传播适应性高于通过血液接触传播的T/F HIV-1：T/F包膜上与传播表型相关的独特聚糖谱。
PLoS Pathog. 2025 May 23;21(5):e1013177. doi: 10.1371/journal.ppat.1013177. eCollection 2025 May.
2
Phenotypic Characterization of Subtype A and Recombinant AC Transmitted/Founder Viruses from a Rwandan HIV-1 Heterosexual Transmission Cohort.卢旺达 HIV-1 异性传播队列中 A 亚型和重组 AC 传播/创始病毒的表型特征。
Viruses. 2024 Oct 30;16(11):1706. doi: 10.3390/v16111706.
3
Virus-Host Gene Interactions Define HIV-1 Disease Progression.病毒-宿主基因相互作用定义了 HIV-1 的疾病进展。
Curr Top Microbiol Immunol. 2017;407:31-63. doi: 10.1007/82_2017_33.
4
Recent Insights into the HIV/AIDS Pandemic.对艾滋病毒/艾滋病大流行的最新见解
Microb Cell. 2016 Sep 5;3(9):451-475. doi: 10.15698/mic2016.09.529.
5
The HIV-1 transmission bottleneck.人类免疫缺陷病毒1型传播瓶颈
Retrovirology. 2017 Mar 23;14(1):22. doi: 10.1186/s12977-017-0343-8.
6
Tracing HIV-1 transmission: envelope traits of HIV-1 transmitter and recipient pairs.追踪HIV-1传播：HIV-1传播者与接受者配对的包膜特征
Retrovirology. 2016 Sep 5;13(1):62. doi: 10.1186/s12977-016-0299-0.
7
Derivation and Characterization of Pathogenic Transmitted/Founder Molecular Clones from Simian Immunodeficiency Virus SIVsmE660 and SIVmac251 following Mucosal Infection.黏膜感染后从猿猴免疫缺陷病毒SIVsmE660和SIVmac251衍生并鉴定致病性传播/奠基分子克隆
J Virol. 2016 Sep 12;90(19):8435-53. doi: 10.1128/JVI.00718-16. Print 2016 Oct 1.
8
Differences in the Selection Bottleneck between Modes of Sexual Transmission Influence the Genetic Composition of the HIV-1 Founder Virus.性传播模式之间选择瓶颈的差异影响HIV-1原始病毒的基因组成。
PLoS Pathog. 2016 May 10;12(5):e1005619. doi: 10.1371/journal.ppat.1005619. eCollection 2016 May.
9
Heterosexual Transmission of Subtype C HIV-1 Selects Consensus-Like Variants without Increased Replicative Capacity or Interferon-α Resistance.C型HIV-1的异性传播选择了类似共识的变体，但其复制能力或对α干扰素的抗性并未增加。
PLoS Pathog. 2015 Sep 17;11(9):e1005154. doi: 10.1371/journal.ppat.1005154. eCollection 2015 Sep.
10
Phenotypic Correlates of HIV-1 Macrophage Tropism.HIV-1巨噬细胞嗜性的表型关联
J Virol. 2015 Nov;89(22):11294-311. doi: 10.1128/JVI.00946-15. Epub 2015 Sep 2.
本文引用的文献
1
Human immunodeficiency virus type 1 V1-to-V5 envelope variants from the chronic phase of infection use CCR5 and fuse more efficiently than those from early after infection.来自感染慢性期的1型人类免疫缺陷病毒V1至V5包膜变体利用CCR5，并且比感染后早期的变体更有效地融合。
J Virol. 2009 Oct;83(19):9694-708. doi: 10.1128/JVI.00925-09. Epub 2009 Jul 22.
2
Heterosexual transmission of human immunodeficiency virus type 1 subtype C: Macrophage tropism, alternative coreceptor use, and the molecular anatomy of CCR5 utilization.人类免疫缺陷病毒1型C亚型的异性传播：巨噬细胞嗜性、替代共受体使用及CCR5利用的分子剖析
J Virol. 2009 Aug;83(16):8208-20. doi: 10.1128/JVI.00296-09. Epub 2009 Jun 10.
3
Appreciating HIV type 1 diversity: subtype differences in Env.认识HIV-1的多样性：Env中的亚型差异。
AIDS Res Hum Retroviruses. 2009 Mar;25(3):237-48. doi: 10.1089/aid.2008.0219.
4
Inflammatory genital infections mitigate a severe genetic bottleneck in heterosexual transmission of subtype A and C HIV-1.炎症性生殖器感染减轻了A和C亚型HIV-1异性传播中的严重基因瓶颈。
PLoS Pathog. 2009 Jan;5(1):e1000274. doi: 10.1371/journal.ppat.1000274. Epub 2009 Jan 23.
5
Selection of HIV variants with signature genotypic characteristics during heterosexual transmission.在异性传播过程中具有特征性基因型特征的HIV变异株的选择。
J Infect Dis. 2009 Feb 15;199(4):580-9. doi: 10.1086/596557.
6
Env length and N-linked glycosylation following transmission of human immunodeficiency virus Type 1 subtype B viruses.1型人类免疫缺陷病毒B亚型病毒传播后的包膜长度和N-连接糖基化
Virology. 2008 May 10;374(2):229-33. doi: 10.1016/j.virol.2008.01.029. Epub 2008 Mar 7.
7
Selection for human immunodeficiency virus type 1 envelope glycosylation variants with shorter V1-V2 loop sequences occurs during transmission of certain genetic subtypes and may impact viral RNA levels.在某些基因亚型的传播过程中，会出现选择具有较短V1-V2环序列的人类免疫缺陷病毒1型包膜糖基化变体的情况，这可能会影响病毒RNA水平。
J Virol. 2005 May;79(10):6528-31. doi: 10.1128/JVI.79.10.6528-6531.2005.
8
Role of low CD4 levels in the influence of human immunodeficiency virus type 1 envelope V1 and V2 regions on entry and spread in macrophages.低CD4水平在1型人类免疫缺陷病毒包膜V1和V2区域对巨噬细胞进入和传播的影响中的作用。
J Virol. 2005 Apr;79(8):4828-37. doi: 10.1128/JVI.79.8.4828-4837.2005.
9
Determinants of human immunodeficiency virus type 1 baseline susceptibility to the fusion inhibitors enfuvirtide and T-649 reside outside the peptide interaction site.人类免疫缺陷病毒1型对融合抑制剂恩夫韦肽和T-649基线易感性的决定因素位于肽相互作用位点之外。
J Virol. 2004 Jul;78(14):7582-9. doi: 10.1128/JVI.78.14.7582-7589.2004.
10
Envelope-constrained neutralization-sensitive HIV-1 after heterosexual transmission.异性传播后受包膜限制的中和敏感型HIV-1
Science. 2004 Mar 26;303(5666):2019-22. doi: 10.1126/science.1093137.
Zotero 插件