母体饮食补充 α-亚麻酸或 n-3 长链多不饱和脂肪酸对新生大鼠脑胶质细胞磷脂酰乙醇胺和磷脂酰丝氨酸脂肪酸谱的差异影响。
Differential effect of maternal diet supplementation with alpha-Linolenic adcid or n-3 long-chain polyunsaturated fatty acids on glial cell phosphatidylethanolamine and phosphatidylserine fatty acid profile in neonate rat brains.
机构信息
Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland.
出版信息
Nutr Metab (Lond). 2010 Jan 14;7:2. doi: 10.1186/1743-7075-7-2.
BACKGROUND
Dietary long-chain polyunsaturated fatty acids (LC-PUFA) are of crucial importance for the development of neural tissues. The aim of this study was to evaluate the impact of a dietary supplementation in n-3 fatty acids in female rats during gestation and lactation on fatty acid pattern in brain glial cells phosphatidylethanolamine (PE) and phosphatidylserine (PS) in the neonates.
METHODS
Sprague-Dawley rats were fed during the whole gestation and lactation period with a diet containing either docosahexaenoic acid (DHA, 0.55%) and eicosapentaenoic acid (EPA, 0.75% of total fatty acids) or alpha-linolenic acid (ALA, 2.90%). At two weeks of age, gastric content and brain glial cell PE and PS of rat neonates were analyzed for their fatty acid and dimethylacetal (DMA) profile. Data were analyzed by bivariate and multivariate statistics.
RESULTS
In the neonates from the group fed with n-3 LC-PUFA, the DHA level in gastric content (+65%, P < 0.0001) and brain glial cell PE (+18%, P = 0.0001) and PS (+15%, P = 0.0009) were significantly increased compared to the ALA group. The filtered correlation analysis (P < 0.05) underlined that levels of dihomo-gamma-linolenic acid (DGLA), DHA and n-3 docosapentaenoic acid (DPA) were negatively correlated with arachidonic acid (ARA) and n-6 DPA in PE of brain glial cells. No significant correlation between n-3 and n-6 LC-PUFA were found in the PS dataset. DMA level in PE was negatively correlated with n-6 DPA. DMA were found to occur in brain glial cell PS fraction; in this class DMA level was correlated negatively with DHA and positively with ARA.
CONCLUSION
The present study confirms that early supplementation of maternal diet with n-3 fatty acids supplied as LC-PUFA is more efficient in increasing n-3 in brain glial cell PE and PS in the neonate than ALA. Negative correlation between n-6 DPA, a conventional marker of DHA deficiency, and DMA in PE suggests n-6 DPA that potentially be considered as a marker of tissue ethanolamine plasmalogen status. The combination of multivariate and bivariate statistics allowed to underline that the accretion pattern of n-3 LC-PUFA in PE and PS differ.
背景
膳食长链多不饱和脂肪酸（LC-PUFA）对神经组织的发育至关重要。本研究旨在评估妊娠和哺乳期母鼠饮食中补充 n-3 脂肪酸对新生大鼠脑胶质细胞磷脂酰乙醇胺（PE）和磷脂酰丝氨酸（PS）中脂肪酸模式的影响。
方法
Sprague-Dawley 大鼠在整个妊娠和哺乳期均喂食含二十二碳六烯酸（DHA，0.55%）和二十碳五烯酸（EPA，总脂肪酸的 0.75%）或α-亚麻酸（ALA，2.90%）的饮食。在两周龄时，分析新生大鼠胃内容物和脑胶质细胞 PE 和 PS 的脂肪酸和二甲乙缩醛（DMA）谱。数据采用双变量和多变量统计分析。
结果
与 ALA 组相比，n-3 LC-PUFA 喂养组新生大鼠胃内容物中的 DHA 水平（增加 65%，P < 0.0001）和脑胶质细胞 PE（增加 18%，P = 0.0001）和 PS（增加 15%，P = 0.0009）均显著增加。过滤相关性分析（P < 0.05）强调，二高-γ-亚麻酸（DGLA）、DHA 和 n-3 二十二碳五烯酸（DPA）水平与花生四烯酸（ARA）和 n-6 DPA 呈负相关在脑胶质细胞的 PE 中。在 PS 数据集未发现 n-3 和 n-6 LC-PUFA 之间存在显著相关性。PE 中的 DMA 水平与 n-6 DPA 呈负相关。在脑胶质细胞 PS 级分中发现 DMA 存在;在这一类中，DMA 水平与 DHA 呈负相关，与 ARA 呈正相关。
结论
本研究证实，母鼠饮食早期补充 n-3 脂肪酸作为 LC-PUFA 比 ALA 更有效地增加新生大鼠脑胶质细胞 PE 和 PS 中的 n-3。PE 中 n-6 DPA（DHA 缺乏的传统标志物）与 DMA 之间的负相关表明，n-6 DPA 可能被视为组织乙醇胺磷酯体状态的标志物。多变量和双变量统计的结合允许强调 n-3 LC-PUFA 在 PE 和 PS 中的积累模式不同。
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