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衰老和环孢素A对人牙龈中胶原周转的影响。

Effects of aging and cyclosporin a on collagen turnover in human gingiva.

作者信息

Gagliano N, Costa F, Tartaglia G M, Pettinari L, Grizzi F, Sforza C, Portinaro N, Gioia M, Annoni G

机构信息

Department of Human Morphology and Biomedical Sciences "Città Studi", EML-Extracellular Matrix Laboratory, Università degli Studi di Milano, School of Medicine, Milan, Italy.

出版信息

Open Dent J. 2009 Nov 5;3:219-26. doi: 10.2174/1874210600903010219.

DOI:10.2174/1874210600903010219
PMID:20148173
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2817891/
Abstract

BACKGROUND

WE AIMED AT CHARACTERIZING THE AGING GINGIVA ANALYZING: i) collagen content and turnover in human gingival tissues and fibroblasts obtained from healthy young and aging subjects. ii) the effect of cyclosporin A administration in human cultured gingival fibroblasts obtained from aging compared to young subjects.

METHODS

Morphological analysis was performed on haematoxylin-eosin and Sirius red stained paraffin-embedded gingival biopsies from young and aging healthy subjects. The expression of the main genes and proteins involved in collagen turnover were determined by real time PCR, dot blot and SDS-zymography on cultured young and aging gingival fibroblasts, and after cyclosporin A administration.

RESULTS

Our results suggest that in healthy aged people, gingival connective tissue is characterized by a similar collagen content and turnover. Collagen turnover pathways are similarly affected by cyclosporin A treatment in young and aging gingival fibroblasts.

CONCLUSIONS

Cyclosporin A administration affects gingival collagen turnover pathways in young and aging fibroblasts at the same extent, suggesting that during aging cyclosporin A administration is not related to relevant collagen turnover modifications.

摘要

背景

我们旨在通过分析以下内容来表征衰老牙龈：i）从健康年轻和老年受试者获取的人牙龈组织和成纤维细胞中的胶原蛋白含量及更新情况。ii）与年轻受试者相比，环孢素A给药对从老年受试者获取的人培养牙龈成纤维细胞的影响。

方法

对来自年轻和老年健康受试者的苏木精 - 伊红和天狼星红染色的石蜡包埋牙龈活检组织进行形态学分析。通过实时PCR、斑点印迹和SDS - 酶谱法，在培养的年轻和老年牙龈成纤维细胞以及给予环孢素A后，测定参与胶原蛋白更新的主要基因和蛋白质的表达。

结果

我们的结果表明，在健康老年人中，牙龈结缔组织的特征是胶原蛋白含量和更新情况相似。在年轻和老年牙龈成纤维细胞中，环孢素A治疗对胶原蛋白更新途径的影响相似。

结论

环孢素A给药对年轻和老年成纤维细胞中牙龈胶原蛋白更新途径的影响程度相同，这表明在衰老过程中，环孢素A给药与相关的胶原蛋白更新改变无关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecd1/2817891/3cbad715ed2e/TODENTJ-3-219_F4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecd1/2817891/6738baebd43f/TODENTJ-3-219_F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecd1/2817891/6332c4278caf/TODENTJ-3-219_F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecd1/2817891/201a4e96c6c8/TODENTJ-3-219_F3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecd1/2817891/3cbad715ed2e/TODENTJ-3-219_F4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecd1/2817891/6738baebd43f/TODENTJ-3-219_F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecd1/2817891/6332c4278caf/TODENTJ-3-219_F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecd1/2817891/201a4e96c6c8/TODENTJ-3-219_F3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecd1/2817891/3cbad715ed2e/TODENTJ-3-219_F4.jpg

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