Hannoun Antoine, Shehab Marwa, Khairallah Marie-Therese, Sabra Ahmad, Abi-Rached Roland, Bazi Tony, Yunis Khalid A, Araj George F, Matar Ghassan M
Department of Obstetrics and Gynecology, Faculty of Medicine, American University of Beirut, 11072020 Beirut, Lebanon.
Int J Microbiol. 2009;2009:796512. doi: 10.1155/2009/796512. Epub 2010 Feb 2.
The antimicrobial susceptibility profiles of 76 Streptococcus agalactiae (Group B Streptococci [GBS]) isolates from vaginal specimens of pregnant women near term were correlated to their genotypes generated by Random Amplified Polymorphic DNA analysis and their virulence factors encoding genes cylE, lmb, scpB, rib, and bca by PCR. Based on the distribution of the susceptibility patterns, six profiles were generated. RAPD analysis detected 7 clusters of genotypes. The cylE gene was present in 99% of the isolates, the lmb in 96%, scpB in 94.7%, rib in 33%, and bca in 56.5% of isolates. The isolates demonstrated a significant correlation between antimicrobial resistance and genotype clusters denoting the distribution of particular clones with different antimicrobial resistance profiles, entailing the practice of caution in therapeutic options. All virulence factors encoding genes were detected in all seven genotypic clusters with rib and bca not coexisting in the same genome.
对76株从近期孕妇阴道标本中分离出的无乳链球菌（B组链球菌[GBS]）进行抗菌药敏分析，并将其与通过随机扩增多态性DNA分析产生的基因型以及通过聚合酶链反应检测到的毒力因子编码基因cylE、lmb、scpB、rib和bca相关联。根据药敏模式的分布，生成了六种谱型。随机扩增多态性DNA分析检测到7个基因型簇。99%的分离株存在cylE基因，96%存在lmb基因，94.7%存在scpB基因，33%存在rib基因，56.5%存在bca基因。分离株显示出抗菌耐药性与基因型簇之间存在显著相关性，这表明具有不同抗菌耐药谱型的特定克隆的分布情况，在治疗选择时需要谨慎。在所有七个基因型簇中均检测到所有毒力因子编码基因，且rib和bca不在同一基因组中共存。
