具有催化活性的人端粒酶中的 RNA 构象。
RNA conformation in catalytically active human telomerase.
机构信息
The University Chemical Laboratory, University of Cambridge, UK.
出版信息
J Am Chem Soc. 2010 Mar 10;132(9):2852-3. doi: 10.1021/ja909383n.
Abstract
We have used single-molecule fluorescence microscopy to study the folded state of human telomerase RNA (hTR). Here we show that hTR adopts a new conformation on binding to human telomerase reverse transcriptase (hTERT) and reconstitution of an active ribonucleoprotein complex. Our data are consistent with the formation of an RNA pseudoknot in active human telomerase.
摘要
我们使用单分子荧光显微镜研究了人类端粒酶 RNA（hTR）的折叠状态。在这里，我们表明 hTR 在与人类端粒酶逆转录酶（hTERT）结合并重新形成活性核糖核蛋白复合物时会采用新的构象。我们的数据与活性人类端粒酶中 RNA 假结的形成一致。
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