Zhang YanLiang, Dai Yong, Huang YuanShuai, Ma Liguo, Yin Yibing, Tang Min, Hu Chengxiao
Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing, China.
J Obstet Gynaecol Res. 2009 Oct;35(5):842-9. doi: 10.1111/j.1447-0756.2009.01055.x.
Micro-ribonucleic acid (miRNA) are noncoding RNA molecules of 21 to 24 nt that regulate the expression of target genes in a post-transcriptional manner. Evidence indicates that miRNA play essential roles in embryogenesis, cell differentiation and pathogenesis of human diseases. This study describes a comparison between the microRNA profile of human-papillomavirus-negative cervical squamous cell carcinoma patients and controls, in order to develop further understanding of the pathogenesis of cervical squamous cell carcinomas.
MiRNA were isolated from tumor tissues of five human-papillomavirus-negative cervical squamous cell carcinoma patients and five healthy controls in order to perform miRNA microarray chip analysis. The chip results were then confirmed by northern blot analysis.
A total of 27 miRNA differentially expressed between the squamous cell carcinoma patients and the healthy controls were identified.
This work indicates that these miRNA may be potential diagnosis biomarkers and probable factors involved in the pathogenesis of cervical squamous cell carcinomas.
微小核糖核酸（miRNA）是21至24个核苷酸的非编码RNA分子，以转录后方式调节靶基因的表达。有证据表明，miRNA在胚胎发生、细胞分化和人类疾病发病机制中起重要作用。本研究对人乳头瘤病毒阴性宫颈鳞状细胞癌患者和对照组的微小RNA谱进行比较，以进一步了解宫颈鳞状细胞癌的发病机制。
从5名人乳头瘤病毒阴性宫颈鳞状细胞癌患者的肿瘤组织和5名健康对照中分离miRNA，以进行miRNA微阵列芯片分析。然后通过Northern印迹分析确认芯片结果。
共鉴定出27种在鳞状细胞癌患者和健康对照之间差异表达的miRNA。
这项工作表明，这些miRNA可能是潜在的诊断生物标志物，也是宫颈鳞状细胞癌发病机制中的可能因素。
