Department of Biochemistry and Molecular Biology, Cancer Institute, Capital Medical University, Beijing, China.
Gynecol Oncol. 2011 Jan;120(1):145-51. doi: 10.1016/j.ygyno.2010.09.009. Epub 2010 Oct 13.
MicroRNA (miRNA) plays an essential role in the progression of a variety of cancers, but its role in cervical cancer progression is not well defined. We aimed to test whether special miRNAs and their target mRNAs contribute to cervical cancer progression.
The expression profiles of 1145 microRNAs in cervical squamous cell carcinomas (CSCC) and adjacent non-tumor tissues were investigated using an Illumina microRNA microarray platform. Differentially expressed miRNAs were validated by RT-PCR. Downstream target validation was performed for miR-886-5p.
We found that the expression levels of seven miRNAs differed significantly between CSCC tissues and adjacent non-tumor tissues. Forced expression of one miRNA, miR-886-5p, over-expressed in CSCC tissues lowered expression of the pro-apoptotic protein Bax, reduced apoptosis and promoted cell proliferation in H8, an HPV16-immortalized human cervical squamous epithelial cell line. Knockdown of miR-886-5p increased Bax protein and apoptotic cell death in cells of the cervical squamous carcinoma cell line, SiHa.
MicroRNA miR-886-5p inhibits apoptosis of cervical cancer cells by down-regulating the production of Bax.
微小 RNA（miRNA）在多种癌症的进展中起着至关重要的作用，但它在宫颈癌进展中的作用尚未明确。我们旨在研究特殊的 miRNA 及其靶 mRNA 是否有助于宫颈癌的进展。
使用 Illumina miRNA 微阵列平台检测 1145 种 miRNA 在宫颈鳞状细胞癌（CSCC）和相邻非肿瘤组织中的表达谱。通过 RT-PCR 验证差异表达的 miRNA。对 miR-886-5p 进行下游靶标验证。
我们发现，CSCC 组织和相邻非肿瘤组织之间七种 miRNA 的表达水平有显著差异。在 HPV16 永生化的人宫颈鳞状上皮细胞系 H8 中，过表达在 CSCC 组织中高表达的一种 miRNA，miR-886-5p，降低了促凋亡蛋白 Bax 的表达，减少了细胞凋亡并促进了细胞增殖。在宫颈鳞状癌细胞系 SiHa 中，下调 miR-886-5p 增加了 Bax 蛋白和凋亡细胞死亡。
miR-886-5p 通过下调 Bax 的产生抑制宫颈癌细胞的凋亡。
