Département de Toxicologie, Centre de Recherches du Service de Santé des Armées (CRSSA), 24 Avenue des maquis du Grésivaudan, 38702 La Tronche Cedex, France.
Chem Biol Interact. 2010 Sep 6;187(1-3):393-6. doi: 10.1016/j.cbi.2010.02.001. Epub 2010 Feb 10.
Degradation of organophosphorus compounds was achieved in the presence of purified fungal laccase from Trametes versicolor and a small molecular weight redox mediator (ABTS). This laccase-mediator system (LMS) catalyzed degradation of VX, PhX and VR while had no apparent effect on CVX, ecothiophate or demeton. Inhibition of ABTS oxidation was shown with VX, PhX, VR and CVX. Results with CVX suggest either no degradation subsequent to interaction with the laccase active site or the formation of a new toxic compound. PhX degradation was also monitored by mass spectroscopy, a method that allowed us to identify certain intermediates formed during OP degradation. Altogether, results underline the importance of the OP nitrogen atom at beta-position and of its substituents, even though the intimate mechanism of laccase-catalyzed degradation is not yet known.
在纯化的来自彩绒革盖菌的漆酶和一种小分子氧化还原介体（ABTS）存在下，实现了有机磷化合物的降解。该漆酶-介体系统（LMS）催化VX、PhX 和 VR 的降解，而对 CVX、乙硫磷或乐果没有明显影响。VX、PhX、VR 和 CVX 对 ABTS 氧化有抑制作用。用 CVX 进行的结果表明，与漆酶活性位点相互作用后要么没有降解，要么形成了一种新的有毒化合物。PhX 的降解也通过质谱法进行了监测，该方法使我们能够鉴定在 OP 降解过程中形成的某些中间产物。总的来说，结果强调了 OP 中β位氮原子及其取代基的重要性，尽管漆酶催化降解的具体机制尚不清楚。
