Departament d'Enginyeria Química, Institut de Ciència i Tecnologia Ambiental, Universitat Autònoma de Barcelona (UAB), 08193 Bellaterra, Spain.
Bioresour Technol. 2010 Apr;101(7):2159-66. doi: 10.1016/j.biortech.2009.11.019. Epub 2009 Dec 8.
The white-rot fungus Trametes vesicolor degraded naproxen (10 mg L(-1)) in a liquid medium to non-detectable levels after 6h. When naproxen was added in the range of concentrations typically found in the environment (55 microg L(-1)), it was almost completely degraded (95%) after 5h. In vitro degradation experiments with purified laccase and purified laccase plus mediator 1-hydroxybenzotriazol showed slight and almost complete naproxen degradation, respectively. A noticeable inhibition on naproxen degradation was also observed when the cytochrome P450 inhibitor 1-aminobenzotriazole was added to the fungal cultures. These data suggest that both enzymatic systems could play a role in naproxen degradation. 2-(6-hydroxynaphthalen-2-yl)propanoic acid and 1-(6-methoxynaphthalen-2-yl)ethanone were structurally elucidated by HPLC-DAD-MS and NMR as degradation intermediates of naproxen. After 6h of incubation, both parent compound and intermediates disappeared from the medium. The non-toxicity of the treated medium was confirmed by Microtox test.
白腐真菌云芝在液体培养基中 6 小时内将萘普生（10mg/L）降解至无法检出的水平。当萘普生的浓度范围在环境中通常存在的浓度范围内（55μg/L）时，5 小时后几乎完全降解（95%）。用纯化漆酶和纯化漆酶加介体 1-羟基苯并三唑进行的体外降解实验分别显示出轻微和几乎完全的萘普生降解。当向真菌培养物中添加细胞色素 P450 抑制剂 1-氨基苯并三唑时，也观察到对萘普生降解的明显抑制。这些数据表明，两种酶系统都可能在萘普生降解中发挥作用。2-(6-羟基萘-2-基)丙酸和 1-(6-甲氧基萘-2-基)乙酮通过 HPLC-DAD-MS 和 NMR 结构鉴定为萘普生的降解中间产物。孵育 6 小时后，母体化合物和中间产物均从培养基中消失。通过 Microtox 试验证实处理后的培养基无毒性。
