酸橙汁（Citrus aurantifolia）对 Sprague-Dawley 大鼠发情周期和排卵的影响。

Effect of the juice of lime (Citrus aurantifolia) on estrous cycle and ovulation of Sprague-Dawley rats.

机构信息

Department of Anatomy, College of Medicine, University of Lagos, Idi-Araba, Lagos, Nigeria.

出版信息

Endocr Pract. 2010 Jul-Aug;16(4):561-5. doi: 10.4158/EP09017.OR.

DOI:10.4158/EP09017.OR

PMID:20150029

Abstract

OBJECTIVE

To determine the effect of lime juice on the estrous cycle and ovulation of cyclic female rats.

METHODS

Twenty-five adult female Sprague-Dawley rats were used. The study was divided into 2 experiments (I and II). In experiment I, 15 rats were randomly subclassified into 3 groups (Ia, Ib, and Ic) of 5 rats each. The estrous cycles of the rats were studied for the first 16 days to establish cyclicity, after which lime juice was administered by gastric gavage for the next 24 days. Rats in group Ia received 1 mL of undiluted lime juice, rats in group Ib received 1 mL of 50% diluted lime juice, and rats in group Ic (control animals) received only distilled water. In experiment II, 10 female rats were used and were categorized into 2 groups (IIa and IIb), with 5 rats in each group. Rats in group IIa received 1 mL of undiluted lime juice during the morning of proestrus, and those in group IIb received only distilled water on the day of proestrus. The rats were killed the next day with use of chloroform anesthesia. The upper parts of the oviducts were excised and examined under the light microscope for assessment of the number of ova shed.

RESULTS

There was an irregular pattern in all phases of the estrous cycle of 100% of the rats given undiluted lime juice and in 80% of those given 50% diluted lime juice. There was a significant (P = .001) reduction in the number of ova shed in rats administered undiluted lime juice in comparison with the control animals. Ovulation was partially blocked, as shown by the reduced number of ova observed in the oviducts from the rats given undiluted lime juice (5.10 +/- 2.37) in comparison with the control rats (12.70 +/- 1.14).

CONCLUSION

In rats, lime juice causes irregularity of the estrous cycle, partially blocks ovulation, and may possibly compromise fertility.

摘要

目的

确定酸橙汁对发情周期和排卵的影响循环雌性大鼠。

方法

使用 25 只成年雌性 Sprague-Dawley 大鼠。研究分为 2 个实验（I 和 II）。在实验 I 中，将 15 只大鼠随机细分为 3 组（Ia、Ib 和 Ic），每组 5 只。研究大鼠的发情周期 16 天以建立周期性，然后用灌胃法在下一个 24 天内给予酸橙汁。组 Ia 的大鼠给予 1 mL 未稀释的酸橙汁，组 Ib 的大鼠给予 1 mL 50%稀释的酸橙汁，组 Ic（对照动物）仅给予蒸馏水。在实验 II 中，使用 10 只雌性大鼠分为 2 组（IIa 和 IIb），每组 5 只。组 IIa 的大鼠在发情前期的早晨给予 1 mL 未稀释的酸橙汁，组 IIb 的大鼠在发情期当天仅给予蒸馏水。第二天，用氯仿麻醉处死大鼠。取出输卵管上部，在光镜下检查评估排卵的卵子数量。

结果

100%给予未稀释酸橙汁的大鼠和 80%给予 50%稀释酸橙汁的大鼠的发情周期各期均出现不规则模式。与对照组相比，给予未稀释酸橙汁的大鼠排卵的卵子数量明显减少（P =.001）。排卵被部分阻断，如从给予未稀释酸橙汁的大鼠的输卵管中观察到的卵子数量减少（5.10 +/- 2.37）与对照组大鼠（12.70 +/- 1.14）相比。