Adams Kelly L, Puchades Maja, Ewing Andrew G
Pennsylvania State University, Department of Chemistry, University Park, Pennsylvania 16802.
Annu Rev Anal Chem (Palo Alto Calif). 2008 Jul 1;1:329. doi: 10.1146/annurev.anchem.1.031207.113038.
This article reviews recent work involving electrochemical methods for in vitro analysis of biomolecules, with an emphasis on detection and manipulation at and of single cells and cultures of cells. The techniques discussed include constant potential amperometry, chronoamperometry, cellular electroporation, scanning electrochemical microscopy, and microfluidic platforms integrated with electrochemical detection. The principles of these methods are briefly described, followed in most cases with a short description of an analytical or biological application and its significance. The use of electrochemical methods to examine specific mechanistic issues in exocytosis is highlighted, as a great deal of recent work has been devoted to this application.
本文综述了近期有关生物分子体外分析电化学方法的研究工作，重点关注单细胞及细胞培养物的检测与操控。所讨论的技术包括恒电位安培法、计时电流法、细胞电穿孔、扫描电化学显微镜以及集成电化学检测的微流控平台。简要描述了这些方法的原理，多数情况下还简短介绍了其分析或生物学应用及其意义。特别强调了利用电化学方法研究胞吐作用中的特定机制问题，因为近期大量工作都致力于此应用。
