Department of Functional Materials and Electronics, State Research Institute Centre for Physical Sciences and Technology, Sauletekio Ave. 3, LT-10257 Vilnius, Lithuania.
Department of Physical Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, Lithuania.
Int J Mol Sci. 2021 May 10;22(9):5032. doi: 10.3390/ijms22095032.
The review focuses on the overview of electrochemical sensors based on molecularly imprinted polymers (MIPs) for the determination of uric acid. The importance of robust and precise determination of uric acid is highlighted, a short description of the principles of molecular imprinting technology is presented, and advantages over the others affinity-based analytical methods are discussed. The review is mainly concerned with the electro-analytical methods like cyclic voltammetry, electrochemical impedance spectroscopy, amperometry, etc. Moreover, there are some scattered notes to the other electrochemistry-related analytical methods, which are capable of providing additional information and to solve some challenges that are not achievable using standard electrochemical methods. The significance of these overviewed methods is highlighted. The overview of the research that is employing MIPs imprinted with uric acid is mainly targeted to address these topics: (i) type of polymers, which are used to design uric acid imprint structures; (ii) types of working electrodes and/or other parts of signal transducing systems applied for the registration of analytical signal; (iii) the description of the uric acid extraction procedures applied for the design of final MIP-structure; (iv) advantages and disadvantages of electrochemical methods and other signal transducing methods used for the registration of the analytical signal; (vi) overview of types of interfering molecules, which were analyzed to evaluate the selectivity; (vi) comparison of analytical characteristics such as linear range, limits of detection and quantification, reusability, reproducibility, repeatability, and stability. Some insights in future development of uric acid sensors are discussed in this review.
本文主要综述了基于分子印迹聚合物(MIP)的电化学传感器用于尿酸测定的研究进展。强调了准确测定尿酸的重要性,介绍了分子印迹技术的基本原理,并讨论了其相对于其他基于亲和力的分析方法的优势。综述内容主要涉及循环伏安法、电化学阻抗谱、电流测定法等电化学分析方法。此外,还对一些其他与电化学相关的分析方法进行了简要介绍,这些方法能够提供更多信息并解决使用标准电化学方法无法解决的一些挑战。文中还强调了这些方法的重要性。本文综述了采用尿酸印迹的 MIP 进行的研究,主要针对以下几个方面:(i)用于设计尿酸印迹结构的聚合物类型;(ii)用于记录分析信号的工作电极和/或其他信号转换系统部分的类型;(iii)用于设计最终 MIP 结构的尿酸提取程序的描述;(iv)用于记录分析信号的电化学方法和其他信号转换方法的优缺点;(vi)分析干扰分子类型的概述,以评估选择性;(vi)比较分析特性,如线性范围、检测限和定量限、可重复性、重现性和稳定性。本文讨论了尿酸传感器未来的发展方向。
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