Almeida Fernando Gonçalves, Nobre Yuri Tulio Dantas, Leite Katia R, Bruschini Homero
Department of Urology, Paulista School of Medicine, Federal University of Sao Paulo, Sao Paulo, Brazil.
Int Urogynecol J. 2010 Jun;21(6):743-8. doi: 10.1007/s00192-009-1090-8. Epub 2010 Feb 12.
A study was carried out to evaluate the feasibility of autologous adipose derived stem cells (ADSC) transplantation into female rabbits' urethra walls as an alternative to intrinsic urethral regeneration.
Inguinal fat pad of 12 New Zealand adult female rabbits were harvested and processed to obtain stromal vascular fraction (SVF). The SVF were platted to isolate ADSC. Before urethral injection, cells were labeled with DiI marker. The urethra wall was injected with 1 x 10(7) autologous cells or saline (sham). The urethra was harvested at 2, 4, and 8 weeks to identify DiI-labeled cells.
At 2 and 4 weeks, the ADSCs create a nodule localized in the urethral sub-mucosa. At 8 weeks, the ADSCs spread and integrated with the urethra wall from the initial injection site.
This is the first study to demonstrate a successful autologous ADSCs transplantation. It confirms that ADSCs can survive and integrate within the urethral wall.
开展了一项研究，以评估将自体脂肪来源干细胞（ADSC）移植到雌性兔尿道壁作为尿道自身再生替代方法的可行性。
采集12只成年新西兰雌性兔的腹股沟脂肪垫并进行处理，以获得基质血管成分（SVF）。将SVF接种以分离ADSC。在尿道注射前，用DiI标记物标记细胞。向尿道壁注射1×10⁷个自体细胞或生理盐水（假手术组）。在第2、4和8周时采集尿道，以鉴定DiI标记的细胞。
在第2周和第4周时，ADSC在尿道黏膜下层形成一个结节。在第8周时，ADSC从初始注射部位扩散并与尿道壁整合。
这是第一项证明自体ADSC移植成功的研究。它证实了ADSC能够在尿道壁内存活并整合。
