Division of Pediatric Oncology, Hematology and Marrow Transplantation, Department of Medical and Surgical Sciences for Children & Adults, University of Modena and Reggio Emilia, Modena Policlinic, Modena, 41100, Italy.
Apoptosis. 2013 Oct;18(10):1274-89. doi: 10.1007/s10495-013-0878-7.
Autologous fat transfer (AFT) is a procedure for adipose tissue (AT) repair after trauma, burns, post-tumor resections and lipodystrophies still negatively impacted by the lack of graft persistence. The reasons behind this poor outcome are unclear and seem to involve damages in either harvested/transplanted mature adipocytes or on their mesenchymal progenitors, namely adipose stromal/stem cells (ASC), and due to post-transplant AT apoptosis and involution. A rabbit subcutaneous AT regeneration model was here developed to first evaluate graft quality at different times after implant focusing on related parameters, such as necrosis and vasculogenesis. Standard AFT was compared with a strategy where purified autologous ASC, combined with hyaluronic acid (HA), assisted AFT. Five million of autologous ex vivo isolated CD29+, CD90+, CD49e+ ASC, loaded into HA, enriched 1 ml of AT generating an early significant protective effect in reducing AFT necrosis and increasing vasculogenesis with a preservation of transplanted AT architecture. This beneficial impact of ASC assisted AFT was then confirmed at three months with a robust lipopreservation and no signs of cellular transformation. By a novel ASC assisted AFT approach we ensure a reduction in early cell death favoring an enduring graft performance possibly for a more stable benefit in patients.
自体脂肪移植（AFT）是一种用于修复创伤、烧伤、肿瘤切除后和脂肪营养不良后脂肪组织（AT）的方法，但仍然受到移植物持久性缺乏的影响。造成这种不良结果的原因尚不清楚，似乎涉及到收获/移植的成熟脂肪细胞或其间充质祖细胞（即脂肪基质/干细胞（ASC））的损伤，以及移植后 AT 细胞凋亡和萎缩。本研究建立了一种兔皮下 AT 再生模型，首先在植入后不同时间评估移植物质量，重点关注相关参数，如坏死和血管生成。将标准 AFT 与一种策略进行了比较，即将纯化的自体 ASC 与透明质酸（HA）结合，辅助 AFT。将 500 万个离体分离的 CD29+、CD90+、CD49e+ ASC 加载到 HA 中，可生成 1 毫升 AT，这在减少 AFT 坏死和增加血管生成方面具有早期显著的保护作用，同时保持移植 AT 的结构。这种 ASC 辅助 AFT 的有益作用在三个月时得到了进一步证实，具有强大的脂肪保存作用，没有细胞转化的迹象。通过一种新型的 ASC 辅助 AFT 方法，我们确保减少早期细胞死亡，有利于持久的移植物性能，从而为患者带来更稳定的益处。
