Mano H, Chermann J C
Unite de Recherches Inserm U 322 sur les Retrovirus et Maladies Associees, Campus Universitaire de Luminy, Marseille, France.
AIDS Res Hum Retroviruses. 1991 Jan;7(1):83-8. doi: 10.1089/aid.1991.7.83.
Human immunodeficiency virus type 1 (HIV-1) infection in utero was examined by isolating the virus and detecting the HIV-1 DNA sequence from different fetal tissues. The brain, thymus, lung, liver, spleen, and placenta tissues from fetuses (10-23 weeks of gestation) born to HIV-1-infected asymptomatic mothers were examined. HIV-1 was isolated from 2 of 7, 1 of 7, and 1 of 7 cocultures of splenic, thymic, and trypsin-resistant cells from the liver and placenta, respectively, with peripheral blood mononuclear cells; 20-30% and 40-60% of splenic and of thymic cells were CD4+ lymphoid cells and 40-80% of trypsin-resistant cells were mononuclear phagocytes. The HIV-1 DNA sequence was detected in 4 of 7, 3 of 7, 1 of 7, 1 of 7, 2 of 7, and 2 of 6 samples from the spleen, thymus, brain, lung, liver, and placenta, respectively, using the polymerase chain reaction. In one case, the intensity of the HIV-1 DNA sequence appeared to be correlated with the success of viral isolation. We indicate that fetal HIV-1 infection may frequently occur in the second trimester and the cells responsible for the infection may be CD4+ lymphoid cells and mononuclear phagocytes.
通过从不同胎儿组织中分离病毒并检测HIV-1 DNA序列，对子宫内的1型人类免疫缺陷病毒（HIV-1）感染情况进行了检查。对HIV-1感染的无症状母亲所生胎儿（妊娠10至23周）的脑、胸腺、肺、肝、脾和胎盘组织进行了检查。分别从7份脾脏、胸腺以及肝脏和胎盘的胰蛋白酶抗性细胞与外周血单核细胞的共培养物中，有2份、1份和1份分离出了HIV-1；脾脏和胸腺细胞中20%-30%以及胰蛋白酶抗性细胞中40%-80%为CD4+淋巴细胞，40%-80%的胰蛋白酶抗性细胞为单核吞噬细胞。使用聚合酶链反应分别在7份脾脏样本中的4份、7份胸腺样本中的3份、7份脑样本中的1份、7份肺样本中的1份、7份肝脏样本中的2份以及6份胎盘样本中的2份中检测到了HIV-1 DNA序列。在一个病例中，HIV-1 DNA序列的强度似乎与病毒分离的成功与否相关。我们指出，胎儿HIV-1感染可能在妊娠中期频繁发生，而导致感染的细胞可能是CD4+淋巴细胞和单核吞噬细胞。
