Kavli Institute of NanoScience, Delft University of Technology, Delft, The Netherlands.
Nano Lett. 2010 Mar 10;10(3):765-72. doi: 10.1021/nl902228p.
DNA molecules can be concentrated in a narrow region of a nanochannel when driven electrokinetically in submillimolar salt solutions. Transport experiments and theoretical modeling reveal the interplay of electrophoresis, electro-osmosis, and the unique statistical properties of confined polymers that lead to DNA aggregation. A finite conductance through the bulk of the device also plays a crucial role by influencing the electric fields in the nanochannel. We build on this understanding by demonstrating how a nanofluidic device with integrated electrodes can preconcentrate DNA at selected locations and at physiological salt concentrations that are relevant to lab-on-a-chip applications.
当 DNA 分子在亚毫摩尔盐溶液中电动力学驱动时，可以在纳米通道的狭窄区域内浓缩。传输实验和理论建模揭示了电泳、电渗流和受限聚合物的独特统计特性之间的相互作用，导致 DNA 聚集。通过影响纳米通道中的电场，器件本体的有限电导率也起着至关重要的作用。我们通过演示具有集成电极的纳流控装置如何在与芯片实验室应用相关的生理盐浓度下在选定位置预浓缩 DNA，进一步加深了对此的理解。
