Mancini R, Paolucci F, Svegliati Baroni G, Jezequel A M, Orlandi F
Cell Pathology Unit, University of Ancona, Italy.
Int J Exp Pathol. 1991 Apr;72(2):119-28.
The present study is concerned with the immunohistochemical characterization in situ of the mononuclear infiltrate accompanying the formation of septa and the development of cirrhosis in the liver of rats treated with dimethylnitrosamine (DMN), i.p. (10 microliters/kg, 3 days a week for 3 weeks). Monoclonal antibodies against macrophages, pan T cells, T cell subsets and B cells have been applied on cryostat sections of animals given DMN for 7, 14 and 21 days. The maximum increase of macrophages and lymphocytes was observed at days 7 and 14 respectively. At all times T lymphocytes appeared as the major component of the inflammatory infiltrate with a largely predominant population of cytotoxic/suppressor T cells. At day 21, with evidence of nodulation of parenchyma, macrophages levelled off while T cells remained numerous without changes in the inducer-helper T cells/cytotoxic-suppressor T cells ratio which remained always less than 1. B cells were always few. These findings illustrate the early influx of lymphocytes in DMN-induced liver injury and help to define the lymphocyte subsets associated with inflammation and fibrosis in a reproducible animal model.
本研究关注腹腔注射二甲基亚硝胺（DMN，10微升/千克，每周3天，共3周）的大鼠肝脏中，伴随间隔形成和肝硬化发展的单核细胞浸润的原位免疫组织化学特征。针对巨噬细胞、全T细胞、T细胞亚群和B细胞的单克隆抗体已应用于接受DMN处理7天、14天和21天的动物的冷冻切片。巨噬细胞和淋巴细胞的最大增幅分别在第7天和第14天观察到。在所有时间点，T淋巴细胞都是炎性浸润的主要成分，其中细胞毒性/抑制性T细胞占主要群体。在第21天，随着实质结节形成的证据出现，巨噬细胞数量趋于稳定，而T细胞仍然很多，诱导辅助性T细胞/细胞毒性抑制性T细胞的比例没有变化，始终小于1。B细胞一直很少。这些发现说明了淋巴细胞在DMN诱导的肝损伤中的早期流入，并有助于在一个可重复的动物模型中确定与炎症和纤维化相关的淋巴细胞亚群。
