Paolucci F, Mancini R, Marucci L, Benedetti A, Jezequel A M, Orlandi F
Postgraudate School of Gastroenterology, University of Ancona, Italy.
Liver. 1990 Oct;10(5):278-81. doi: 10.1111/j.1600-0676.1990.tb00470.x.
The present study is concerned with changes in the number and localization of S-phase cells in the liver of rats exposed to dimethylnitrosamine (DMN). S-phase cells were detected by immunohistochemistry after injection of bromodeoxyuridine (BrdU) and exposure of paraffin sections of liver tissue to the antibody anti-BrdU. With respect to controls, the number of S-phase cells increased four to fivefold in DMN-treated animals in the first week of treatment and remained significantly higher thereafter, in association with the formation of septa. At all times, the labelling index was higher in littoral cells than in hepatocytes. No labelling was observed in biliary cells. This behaviour is different from that reported in other situations, for instance in regeneration after partial hepatectomy, which suggests that besides hepatocytes and littoral cells replacement, an involvement of the latter cell line in the inflammatory reaction, synthesis of extracellular matrix components and formation of septa may account for this particular pattern.
本研究关注暴露于二甲基亚硝胺（DMN）的大鼠肝脏中S期细胞数量和定位的变化。注射溴脱氧尿苷（BrdU）后，通过免疫组织化学检测S期细胞，将肝组织石蜡切片与抗BrdU抗体孵育。与对照组相比，在处理的第一周，DMN处理的动物中S期细胞数量增加了四到五倍，此后一直显著高于对照组，同时伴有肝小叶间隔的形成。在所有时间点，边缘细胞的标记指数均高于肝细胞。胆管细胞未观察到标记。这种行为与其他情况不同，例如部分肝切除术后的再生，这表明除了肝细胞和边缘细胞的替代外，后一种细胞系参与炎症反应、细胞外基质成分的合成以及间隔的形成可能是这种特殊模式的原因。
