台湾金线莲对大鼠肝脏纤维化和再生的影响。

Effect of Anoectochilus formosanus on fibrosis and regeneration of the liver in rats.

Shih Chun-Ching, Wu Yueh-Wern, Hsieh Chang-Chi, Lin Wen-Chuan

Graduate Institute of Chinese Pharmaceutical Sciences, China Medical University, Taichung, Taiwan.

Clin Exp Pharmacol Physiol. 2004 Sep;31(9):620-5. doi: 10.1111/j.1440-1681.2004.04062.x.

Abstract

The present study examined the effects of an aqueous extract of Anoectochilus formosanus (AFE) on both hepatic fibrosis and regeneration in rats. 2. Fibrosis was induced by intraperitoneal injection of dimethylnitrosamine (DMN) for 3 consecutive days per week for 4 weeks. 3. In DMN-treated rats, liver cirrhosis-associated complications, such as liver atrophy, low concentrations of serum albumin and the accumulation of hepatic collagen, were observed. The AFE protected the liver against DMN-induced fibrosis, as determined by morphological and biochemical observations. 4. In addition, AFE was administered to two-thirds hepatectomized normal and DMN-injured rats. Three and 5 days after hepatectomy, AFE increased the extent of liver weight regeneration and the number of S-phase cells in DMN-injured rats, but not in normal rats. 5. These results show that AFE seems to be useful in the repair of liver injury, improvement of fibrotic changes and promotion of liver regeneration.

摘要

本研究检测了台湾金线莲水提取物（AFE）对大鼠肝纤维化和肝再生的影响。2. 通过每周连续3天腹腔注射二甲基亚硝胺（DMN），持续4周来诱导肝纤维化。3. 在DMN处理的大鼠中，观察到了肝硬化相关并发症，如肝萎缩、血清白蛋白浓度降低和肝胶原积累。通过形态学和生化观察确定，AFE可保护肝脏免受DMN诱导的纤维化。4. 此外，将AFE给予三分之二肝切除的正常大鼠和DMN损伤大鼠。肝切除术后3天和5天，AFE增加了DMN损伤大鼠的肝脏重量再生程度和S期细胞数量，但对正常大鼠没有影响。5. 这些结果表明，AFE似乎有助于修复肝损伤、改善纤维化变化并促进肝再生。