Leibovici J, Klorin G, Huszar M, Hoenig S, Michowitz M
Department of Pathology, Sackler Faculty of Medicine, Tel-Aviv University, Israel.
Int J Exp Pathol. 1991 Apr;72(2):139-50.
The currently available antitumoral therapeutic modalities are most often inefficient against metastatic disease. The metastatic phenotype has been shown to be largely determined by cell membrane properties. The cell membrane could therefore be considered as a possible target for antimetastatic drugs. In the present study we examined the effect of hyperthermia (the antitumoral effect of which is based, at least partly, on an action on the cell membrane) on the F1 and F10 variants of B16 melanoma. Cells of the more malignant variant, F10, were found to be markedly more sensitive to hyperthermic treatment than those of the less malignant one, F1. One hour in-vitro treatment by supranormal temperatures (ranging from 40 to 46 degrees C) resulted in a differential effect with regard to both proliferating capacity of the cells in vitro and tumorigenic ability following inoculation to mice. Our present results in the B16 melanoma corroborate data obtained by us in another tumour system, the AKR lymphoma. Study of the effect of membrane-acting agents on tumour variants differing in degree of malignancy might result in the finding of antitumoral agents efficient against advanced cancer.
目前可用的抗肿瘤治疗方式通常对转移性疾病疗效不佳。已表明转移表型很大程度上由细胞膜特性决定。因此，细胞膜可被视为抗转移药物的一个可能靶点。在本研究中，我们检测了热疗（其抗肿瘤作用至少部分基于对细胞膜的作用）对B16黑色素瘤F1和F10变体的影响。发现恶性程度更高的F10变体细胞比恶性程度较低的F1变体细胞对热疗明显更敏感。超正常温度（40至46摄氏度）体外处理1小时，对体外细胞增殖能力和接种到小鼠后的致瘤能力均产生了差异效应。我们目前在B16黑色素瘤中的研究结果证实了我们在另一个肿瘤系统AKR淋巴瘤中获得的数据。研究作用于细胞膜的药物对恶性程度不同的肿瘤变体的影响，可能会找到对晚期癌症有效的抗肿瘤药物。
