Klorin G, Siegal A, Bar-Shira-Maymon B, Klein O, Leibovici J
Department of Pathology, Sackler Faculty of Medicine, Tel-Aviv University, Israel.
Int J Exp Pathol. 1990 Aug;71(4):469-77.
The cure of metastatic disease constitutes a serious problem. Recent findings showed cell membrane differences between slightly and highly metastasizing tumour cells, suggesting that since this cell organelle determines the metastatic phenotype, it might serve as a target for future drugs programmed against advanced cancer. The cell membrane has also been shown to be involved in the evolution of drug resistance which often accompanies tumour progression. In the present study, the effect of hyperthermia--an antitumoral treatment modality partly exerting its effect on the cell membrane--on primary and metastatic AKR lymphoma cells was compared. The effect of hyperthermia in conjunction with adriamycin (ADR) on the two cell types was also tested. Hyperthermic treatment, alone and in combination with ADR, was more effective in reducing the tumorigenicity of cells derived from metastatic tumours than of the primary tumour cells. Fluorescent microscopy and cytofluorometry showed that the increased effect of ADR by hyperthermia was due to an increased drug uptake at the supranormal temperature.
转移性疾病的治疗是一个严重的问题。最近的研究结果显示，低度和高度转移的肿瘤细胞之间存在细胞膜差异，这表明由于这种细胞器决定了转移表型，它可能成为未来针对晚期癌症设计的药物的靶点。细胞膜也被证明与肿瘤进展过程中经常伴随的耐药性演变有关。在本研究中，比较了热疗（一种部分作用于细胞膜的抗肿瘤治疗方式）对原发性和转移性AKR淋巴瘤细胞的影响。还测试了热疗联合阿霉素（ADR）对这两种细胞类型的影响。单独使用热疗以及热疗与ADR联合使用时，降低转移性肿瘤来源细胞的致瘤性比降低原发性肿瘤细胞的致瘤性更有效。荧光显微镜检查和细胞荧光测定法表明，热疗增强ADR的效果是由于在超常温度下药物摄取增加。
