Department of Oral Health Promotion, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Japan.
Trials. 2010 Feb 12;11:14. doi: 10.1186/1745-6215-11-14.
Previous research has shown the oxidizing properties and microbiological efficacies of chlorine dioxide (ClO2). Its clinical efficacies on oral malodor have been evaluated and reported only in short duration trials, moreover, no clinical studies have investigated its microbiological efficacies on periodontal and malodorous bacteria. Thus, the aim of this study was to assess the inhibitory effects of a mouthwash containing ClO2 used for 7 days on morning oral malodor and on salivary periodontal and malodorous bacteria.
METHODS/DESIGN: A randomized, double blind, crossover, placebo-controlled trial was conducted among 15 healthy male volunteers, who were divided into 2 groups. Subjects were instructed to rinse with the experimental mouthwash containing ClO2 or the placebo mouthwash, without ClO2, twice per day for 7 days. After a one week washout period, each group then used the opposite mouthwash for 7 days. At baseline and after 7 days, oral malodor was evaluated with Organoleptic measurement (OM), and analyzed the concentrations of hydrogen sulfide (H2S), methyl mercaptan (CH3SH) and dimethyl sulfide ((CH3)2S), the main VSCs of human oral malodor, were assessed by gas chromatography (GC). Clinical outcome variables included plaque and gingival indices, and tongue coating index. The samples of saliva were microbiologically investigated. Quantitative and qualitative analyses were performed using the polymerase chain reaction-Invader method.
The baseline oral condition in healthy subjects in the 2 groups did not differ significantly. After rinsing with the mouthwash containing ClO2 for 7 days, morning bad breath decreased as measured by the OM and reduced the concentrations of H2S, CH3SH and (CH3)2S measured by GC, were found. Moreover ClO2 mouthwash used over a 7-day period appeared effective in reducing plaque, tongue coating accumulation and the counts of Fusobacterium nucleatum in saliva. Future research is needed to examine long-term effects, as well as effects on periodontal diseases and plaque accumulation in a well-defined sample of halitosis patients and broader population samples.
ClinicalTrials.gov NCT00748943.
先前的研究已经表明二氧化氯（ClO2）具有氧化性和微生物功效。其在口腔异味方面的临床疗效仅在短期试验中进行了评估和报告，此外，尚无临床研究调查其对牙周病和有异味细菌的微生物功效。因此，本研究的目的是评估含 ClO2 的漱口液连续使用 7 天对晨口气和唾液牙周病及有异味细菌的抑制作用。
方法/设计：在 15 名健康男性志愿者中进行了一项随机、双盲、交叉、安慰剂对照试验，将他们分为 2 组。受试者被指示每天使用含 ClO2 的实验漱口水或不含 ClO2 的安慰剂漱口水漱口两次，持续 7 天。在一周的洗脱期后，每组再使用相反的漱口水 7 天。在基线和 7 天后，使用嗅觉测量法（OM）评估口腔异味，并通过气相色谱法（GC）分析氢硫化物（H2S）、甲硫醇（CH3SH）和二甲硫醚（（CH3）2S）的浓度，这是人体口腔异味的主要 VSCs。临床结果变量包括牙菌斑和牙龈指数以及舌苔指数。通过聚合酶链反应-Invader 方法对唾液样本进行微生物学调查。使用聚合酶链反应-Invader 方法进行定量和定性分析。
两组健康受试者的基线口腔状况无明显差异。用含 ClO2 的漱口水漱口 7 天后，OM 测量的晨口气变差，GC 测量的 H2S、CH3SH 和（CH3）2S 浓度降低。此外，含 ClO2 的漱口水连续使用 7 天可有效减少牙菌斑、舌苔堆积和唾液中梭杆菌属核的计数。需要进一步研究以检查长期效果，以及对牙周病和口臭患者及更广泛人群样本中牙菌斑积累的影响。
ClinicalTrials.gov NCT00748943。
