Université de Toulouse, UPS, Centre de Biologie du Développement, F-31062, Toulouse, France.
Dev Cell. 2010 Jan 19;18(1):64-76. doi: 10.1016/j.devcel.2009.11.009.
The zona pellucida domain (ZPD) defines a conserved family of membrane-anchored matrix proteins that are, as yet, poorly characterized with respect to their functions during development. Using genetic approaches in flies, we show here that a set of eight ZPD proteins is required for the localized reorganization of embryonic epidermal cells during morphogenesis. Despite varying degrees of sequence conservation, these ZPD proteins exert specific and nonredundant functions in the remodeling of epidermal cell shape. Each one accumulates in a restricted subregion of the apical compartment, where it organizes local interactions between the membrane and the extracellular matrix. In addition, ZPD proteins are required to sculpture the actin-rich cell extensions and maintain appropriate organization of the apical compartment. These results on ZPD proteins therefore reveal a functional subcompartmentalization of the apical membrane and its role in the polarized control of epithelial cell shape during development.
透明带域（ZPD）定义了一组保守的膜锚定基质蛋白，这些蛋白的功能在发育过程中还没有得到很好的描述。通过在果蝇中使用遗传方法，我们在这里表明，一组八个 ZPD 蛋白对于胚胎表皮细胞在形态发生过程中的局部重排是必需的。尽管序列的保守程度不同，但这些 ZPD 蛋白在重塑表皮细胞形状方面具有特定的、非冗余的功能。每一种 ZPD 蛋白都在顶端隔室的一个受限亚区积累，在那里它组织了膜和细胞外基质之间的局部相互作用。此外，ZPD 蛋白还需要塑造富含肌动蛋白的细胞延伸，并维持顶端隔室的适当组织。因此，这些关于 ZPD 蛋白的结果揭示了顶端膜的功能亚区化及其在发育过程中极化控制上皮细胞形状中的作用。
