Institute of Food Safety, Chinese Academy of Inspection and Quarantine, No 3, Gaobeidian North Road, Chaoyang District, Beijing 100123, PR China.
Talanta. 2010 Apr 15;81(1-2):156-61. doi: 10.1016/j.talanta.2009.11.051. Epub 2009 Nov 29.
Molecularly imprinted polymers (MIPs) using methyl-testosterone as the template, methacrylic acid (MAA) as the monomer and ethylene glycol dimethacrylate (EDMA) as the crosslinker were prepared by precipitation polymerization. The morphology of the obtained particles was characterized by scanning electron microscopy (SEM) and the pore size was measured by BET. Then, the specificity and selectivity of the MIPs were evaluated using the equilibrium rebinding experiments. Besides, the MIPs were also used as the stationary phase of HPLC column and the retention behaviour to the template and analogues was confirmed using HPLC-MS-MS. Finally, the real application of the methyl-testosterone imprinted polymers was evaluated using SPE procedure with the spiked tap water and lake water. The results indicated that the prepared methyl-testosterone imprinted polymer showed specific rebinding ability to its template and could retain the template strongly compared with other structural analogues. At the same time, the MIPs could be used as SPE column to enrich methyl-testosterone in the lake water and show broad prospects in real samples.
采用甲基睾酮为模板分子、甲基丙烯酸（MAA）为功能单体、乙二醇二甲基丙烯酸酯（EDMA）为交联剂，通过沉淀聚合法制备了分子印迹聚合物（MIPs）。采用扫描电子显微镜（SEM）对所得粒子的形态进行了表征，并通过 BET 法测量了孔径。然后，通过平衡结合实验评价了 MIPs 的特异性和选择性。此外，还将 MIPs 用作 HPLC 柱的固定相，并通过 HPLC-MS-MS 证实了其对模板和类似物的保留行为。最后，通过 SPE 程序对自来水和湖水进行加标实验，评估了甲基睾酮印迹聚合物的实际应用。结果表明，所制备的甲基睾酮印迹聚合物对其模板具有特异性的结合能力，与其他结构类似物相比，对模板的保留能力较强。同时，MIPs 可作为 SPE 柱用于富集湖水中的甲基睾酮，在实际样品中具有广阔的应用前景。
