Dpto. Química Analítica y Análisis Instrumental, Universidad Autónoma de Madrid, 28049 Madrid, Spain.
Talanta. 2010 Mar 15;80(5):2149-56. doi: 10.1016/j.talanta.2009.11.022. Epub 2009 Nov 12.
This work reports on the performance of carbon nanotube modified screen-printed electrodes (SPE-MWCNT) for the selective determination of dopamine (DA) in the presence of ascorbic acid (AA) by adsorptive stripping voltammetry (AdSV). Several operating conditions and parameters were examined including the electrochemical pre-treatment and the previous AA interaction and DA accumulation in the presence AA at physiological conditions. Under the chosen conditions, DA peak current of differential pulse voltammograms increases linearly with DA concentration in the range of 5.0 x 10(-8) to 1.0 x 10(-6) mol L(-1) with a limit of detection of 1.5 x 10(-8) mol L(-1) in connection with 600s accumulation time. The sensitivity obtained for DA was independent from the presence or absence of AA; therefore, the proposed method can be readily applied to detect DA in real samples. The proposed methodology was successfully used for the quantification of DA in urine samples.
这项工作报道了通过吸附溶出伏安法（AdSV），使用碳纳米管修饰的丝网印刷电极（SPE-MWCNT）在存在抗坏血酸（AA）的情况下选择性测定多巴胺（DA）的性能。研究了包括电化学预处理以及 AA 相互作用和 DA 在生理条件下存在 AA 时的积累在内的多种操作条件和参数。在选定的条件下，差分脉冲伏安法的 DA 峰电流在 5.0 x 10(-8) 至 1.0 x 10(-6) mol L(-1) 的范围内与 DA 浓度呈线性关系，检测限为 1.5 x 10(-8) mol L(-1)，与 600s 的积累时间相关。所得 DA 的灵敏度与 AA 的存在与否无关；因此，该方法可用于检测真实样品中的 DA。所提出的方法成功用于尿液样品中 DA 的定量。
