Büyüktaş Duygu, Ghaani Masoud, Rovera Cesare, Carullo Daniele, Olsson Richard T, Korel Figen, Farris Stefano
Department of Food Engineering, Faculty of Engineering, Izmir Institute of Technology, 35430, Gülbahçe Köyü, Urla, Izmir, Turkey.
DeFENS, Department of Food, Environmental and Nutritional Sciences, Food Packaging Lab., University of Milan, via Celoria 2 - I, 20133, Milan, Italy.
Heliyon. 2023 Apr 6;9(4):e15327. doi: 10.1016/j.heliyon.2023.e15327. eCollection 2023 Apr.
Developing simple, cost-effective, easy-to-use, and reliable analytical devices if of utmost importance for the food industry for rapid in-line checks of their products that must comply with the provisions set by the current legislation. The purpose of this study was to develop a new electrochemical sensor for the food packaging sector. More specifically, we propose a screen-printed electrode (SPE) modified with cellulose nanocrystals (CNCs) and gold nanoparticles (AuNPs) for the quantification of 4,4'-methylene diphenyl diamine (MDA), which is one of the most important PAAs that can transfer from food packaging materials into food stuffs. The electrochemical performance of the proposed sensor (AuNPs/CNCs/SPE) in the presence of 4,4'-MDA was evaluated using cyclic voltammetry (CV). The modified AuNPs/CNCs/SPE showed the highest sensitivity for 4,4'-MDA detection, with a peak current of 9.81 μA compared with 7.08 μA for the bare SPE. The highest sensitivity for 4,4'-MDA oxidation was observed at pH = 7, whereas the detection limit was found at 57 nM and the current response of 4,4'-MDA rose linearly as its concentration increased from 0.12 μM to 100 μM. Experiments using real packaging materials revealed that employing nanoparticles dramatically improved both the sensitivity and the selectivity of the sensor, which can be thus considered as a new analytical tool for quick, simple, and accurate measurement of 4,4'-MDA during converting operations.
开发简单、经济高效、易于使用且可靠的分析设备对于食品行业至关重要，以便对其产品进行快速在线检测，这些产品必须符合现行法规规定。本研究的目的是为食品包装领域开发一种新型电化学传感器。更具体地说，我们提出了一种用纤维素纳米晶体（CNCs）和金纳米颗粒（AuNPs）修饰的丝网印刷电极（SPE），用于定量4,4'-亚甲基二苯胺（MDA），它是最重要的可从食品包装材料转移到食品中的多环芳烃之一。使用循环伏安法（CV）评估了所提出的传感器（AuNPs/CNCs/SPE）在4,4'-MDA存在下的电化学性能。修饰后的AuNPs/CNCs/SPE对4,4'-MDA检测显示出最高灵敏度，其峰值电流为9.81 μA，而裸SPE的峰值电流为7.08 μA。在pH = 7时观察到对4,4'-MDA氧化的最高灵敏度，而检测限为57 nM，并且随着4,4'-MDA浓度从0.12 μM增加到100 μM，其电流响应呈线性上升。使用真实包装材料的实验表明，采用纳米颗粒显著提高了传感器的灵敏度和选择性，因此可将其视为在加工操作过程中快速、简单且准确测量4,4'-MDA的一种新型分析工具。
