National Institute for Research and Development of Isotopic and Molecular Technologies, Donat Street, No. 67-103, RO, 400293 Cluj-Napoca, Romania.
NANOM MEMS srl, G. Cosbuc Street, No. 9, RO, 505400 Rasnov, Brasov, Romania.
Sensors (Basel). 2020 Jun 26;20(12):3609. doi: 10.3390/s20123609.
Flexible screen-printed electrodes (HP) were fabricated on stone paper substrate and amperometrically modified with gold nanoparticles (HP-AuNPs). The modified electrode displayed improved electronic transport properties, reflected in a low charge-transfer resistance (1220 Ω) and high apparent heterogeneous electron transfer rate constant (1.94 × 10 cm/s). The voltammetric detection of dopamine (DA) was tested with HP and HP-AuNPs electrodes in standard laboratory solutions (pH 6 phosphate-buffered saline (PBS)) containing various concentrations of analyte (10-10 M). As expected, the modified electrode exhibits superior performances in terms of linear range (10-10 M) and limit of detection (3 × 10 M), in comparison with bare HP. The determination of DA was tested with HP-AuNPs in spiked artificial urine and in pharmaceutical drug solution (ZENTIVA) that contained dopamine hydrochloride (5 mg/mL). The results obtained indicated a very good DA determination in artificial urine without significant matrix effects. In the case of the pharmaceutical drug solution, the DA determination was affected by the interfering species present in the vial, such as sodium metabisulfite, maleic acid, sodium chloride, and propylene glycol.
在石 paper 基底上制作了柔性丝网印刷电极 (HP)，并用金纳米粒子 (HP-AuNPs) 进行了电流计修饰。修饰后的电极显示出改善的电子传输性能，表现为低电荷转移电阻 (1220 Ω) 和高表观异相电子转移速率常数 (1.94×10-2 cm/s)。在含有各种浓度分析物 (10-10 M) 的标准实验室溶液 (pH 6 磷酸盐缓冲盐水 (PBS)) 中，使用 HP 和 HP-AuNPs 电极测试了多巴胺 (DA) 的伏安检测。如预期的那样，与裸 HP 相比，修饰后的电极在线性范围 (10-10 M) 和检测限 (3×10-7 M) 方面表现出优异的性能。使用 HP-AuNPs 在人工尿液和含有盐酸多巴胺 (5 mg/mL) 的药物溶液 (ZENTIVA) 中测试了 DA 的测定。结果表明，在人工尿液中进行 DA 测定时，没有明显的基质效应。在药物溶液的情况下，DA 的测定受到小瓶中存在的干扰物质的影响，例如亚硫酸氢钠、马来酸、氯化钠和丙二醇。
