Department of Orthodontics and Dentofacial Orthopedics, University of Berne, Berne, Switzerland.
Am J Orthod Dentofacial Orthop. 2010 Feb;137(2):223-8. doi: 10.1016/j.ajodo.2008.03.027.
Fixed orthodontic appliances can alter the subgingival microbiota. Our aim was to compare the subgingival microbiota and clinical parameters in adolescent subjects at sites of teeth treated with orthodontic bands with margins at (OBM) or below the gingival margin (OBSM), or with brackets (OBR).
Microbial samples were collected from 33 subjects (ages, 12-18 years) in treatment more than 6 months. The microbiota was assessed by the DNA-DNA checkerboard hybridization method.
Bacterial samples were taken from 83 OBR,103 OBSM, and 54 OBM sites. Probing pocket depths differed by orthodontic type (P <0.001) with mean values of 2.9 mm (SD, 0.6) at OBSM sites, 2.5 mm (SD, 0.6) at OBM sites, and 2.3 mm (SD, 0.5) at OBR sites. Only Actinomyces israelii (P <0.001) and Actinomyces naeslundii (P <0.001) had higher levels at OBR sites, whereas Neisseria mucosa had higher levels at sites treated with OBSM or OBM (P <0.001). Aggregatibacter actinomycetemcomitans was found in 25% of sites independent of the appliance.
Different types of orthodontic appliances cause minor differences in the subgingival microbiota (A israelii and A naeslundii) and higher levels at sites treated with orthodontic brackets. More sites with bleeding on probing and deeper pockets were found around orthodontic bands.
固定正畸矫治器会改变龈下微生物群。我们的目的是比较青少年患者中接受正畸带（OBM）或龈下边缘（OBSM）治疗的牙齿和接受托槽（OBR）治疗的牙齿的龈下微生物群和临床参数。
从 33 名治疗时间超过 6 个月的受试者中采集微生物样本。采用 DNA-DNA 斑点杂交法评估微生物群。
从 83 个 OBR、103 个 OBSM 和 54 个 OBM 位点采集了细菌样本。正畸类型对探诊袋深度有影响（P<0.001），OBSM 位点的平均深度为 2.9mm（SD，0.6），OBM 位点为 2.5mm（SD，0.6），OBR 位点为 2.3mm（SD，0.5）。只有放线菌属以色列（P<0.001）和放线菌属奈瑟（P<0.001）在 OBR 位点的水平较高，而奈瑟氏菌属在接受 OBSM 或 OBM 治疗的位点水平较高（P<0.001）。无论使用何种矫治器，黏放线菌的检出率均为 25%。
不同类型的正畸矫治器会导致龈下微生物群（A israelii 和 A naeslundii）出现轻微差异，并使接受正畸托槽治疗的位点的水平更高。在接受正畸带治疗的部位，探诊出血和探诊袋更深的部位更多。
