National Institute of Health Doutor Ricardo Jorge, Rua Alexandre Herculano 321, 4000-055 Porto, Portugal; Requimte, University of Porto, Portugal.
Mar Pollut Bull. 2010 Jun;60(6):819-26. doi: 10.1016/j.marpolbul.2010.01.013. Epub 2010 Feb 11.
The increasing demands on recreational waters have made microbial contamination a matter of public and scientific concern. This study aimed to search for Salmonella spp. in waters classified according EU Directive 2006/7/EC, in order to assess associations between its prevalence and the concentration of the non-pathogenic new faecal indicators: Escherichia coli and intestinal enterococci. Although a statistically significant association was observed Salmonella was detected on beaches classified as "Good" (9.3%) and "Excellent" (14.4%) which compromises the idea that faecal indicators can be predictors of pathogens. Attending the high prevalence of Salmonella found (23.1%) it seemed important to improve the efficiency of the conventional analytical method (ISO 6340:1995), comparing its draft with SML-VIDAS Salmonella and two new chromogenic media: AES Salmonella Agar Plate (ASAP) and Simple Method Salmonella (SMS). ASAP showed the higher efficiency and can be recommended for a faster detection and presumptive identification of salmonellae in bathing waters.
休闲水域的需求不断增加,使得微生物污染成为公众和科学界关注的问题。本研究旨在根据欧盟指令 2006/7/EC 对水进行沙门氏菌检测,以评估其流行率与非致病新型粪便指标(大肠杆菌和肠道肠球菌)浓度之间的关联。尽管观察到沙门氏菌与被归类为“良好”(9.3%)和“优秀”(14.4%)的海滩之间存在统计学上的显著关联,但这与粪便指标可以预测病原体的观点相矛盾。鉴于沙门氏菌的高流行率(23.1%),似乎有必要提高传统分析方法(ISO 6340:1995)的效率,将其草案与 SML-VIDAS 沙门氏菌和两种新的显色培养基 AES 沙门氏菌琼脂平板(ASAP)和简易沙门氏菌检测(SMS)进行比较。ASAP 显示出更高的效率,可推荐用于更快地检测和推定鉴定浴场中的沙门氏菌。