Dept. of Applied Physics, Chalmers University of Technology, SE-412 96 Göteborg, Sweden.
Biosens Bioelectron. 2011 Jan 15;26(5):1833-8. doi: 10.1016/j.bios.2010.01.018. Epub 2010 Jan 25.
An electrodeless quartz crystal microbalance with dissipation monitoring (QCM-D) setup is used to monitor the formation of supported lipid bilayers (SLBs) on bare quartz crystal sensor surfaces. The kinetic behavior of the formation of a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) SLB on SiO(2) surfaces is discussed and compared for three cases: (i) a standard SiO(2) film deposited onto the gold electrode of a quartz crystal, (ii) an electrodeless quartz crystal with a sputter-coated SiO(2) film, and (iii) an uncoated electrodeless quartz crystal sensor surface. We demonstrate, supported by imaging the SLB on an uncoated electrodeless surface using atomic force microscopy (AFM), that a defect-free, completely covering bilayer is formed in all three cases. Differences in the kinetics of the SLB formation on the different sensor surfaces are attributed to differences in surface roughness. The latter assumption is supported by imaging the different surfaces using AFM. We show furthermore that electrodeless quartz crystal sensors can be used not only for the formation of neutral SLBs but also for positively and negatively charged SLBs. Based on our results we propose electrodeless QCM-D to be a valuable technique for lipid bilayer and related applications providing several advantages compared to electrode-coated surfaces like optical transparency, longer lifetime, and reduced costs.
采用无电极石英晶体微天平（QCM-D）装置监测在裸石英晶体传感器表面形成支撑脂双层（SLB）的过程。本文讨论并比较了三种情况下 1-棕榈酰-2-油酰-sn-甘油-3-磷酸胆碱（POPC）SLB 在 SiO2 表面上的形成动力学行为：（i）沉积在石英晶体金电极上的标准 SiO2 薄膜，（ii）溅射涂覆 SiO2 薄膜的无电极石英晶体，以及（iii）未涂覆的无电极石英晶体传感器表面。我们通过使用原子力显微镜（AFM）对未涂覆的无电极表面上的 SLB 进行成像，证明在所有三种情况下都形成了无缺陷、完全覆盖的双层，从而证实了这一点。在不同传感器表面上形成 SLB 的动力学差异归因于表面粗糙度的差异。这一假设得到了使用 AFM 对不同表面进行成像的支持。我们还表明，无电极石英晶体传感器不仅可用于中性 SLB 的形成，还可用于正电荷和负电荷 SLB 的形成。基于我们的结果，我们提出无电极 QCM-D 是一种有价值的脂质双层和相关应用技术，与电极涂覆表面相比具有几个优势，如光学透明度、更长的寿命和更低的成本。
