School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea.
Bioresour Technol. 2010 Jun;101(12):4533-9. doi: 10.1016/j.biortech.2010.01.080. Epub 2010 Feb 11.
For the production of poly(gamma-glutamic acid), a newly isolated Bacillus sp. RKY3 was phylogenetically identified as Bacillus subtilis based on its 16S rRNA gene sequence. The culture medium for the production of poly(gamma-glutamic acid) by B. subtilis RKY3 was optimized statistically. The parameters significantly affecting poly(gamma-glutamic acid) production were found to be glycerol, glutamic acid, yeast extract, and K(2)HPO(4). A further advanced statistical approach, central composite design, found the optimum levels of the screened variables as follows (gl(-1)): glycerol 17.6, glutamic acid 59.6; yeast extract 2.7; K(2)HPO(4) 2.3. The predicted response as poly(gamma-glutamic acid) production under the statistically optimized conditions was 48.5 g l(-1), which was only 0.4% different from the maximum poly(gamma-glutamic acid) concentration (48.7 g l(-1)) observed at the validation experiment using 7-l lab-scale fermentor containing 3 l of working volume.
为了生产聚（γ-谷氨酸），新分离的芽孢杆菌 RKY3 根据其 16S rRNA 基因序列被鉴定为枯草芽孢杆菌。通过统计方法优化了生产聚（γ-谷氨酸）的培养基。发现影响聚（γ-谷氨酸）生产的显著参数是甘油、谷氨酸、酵母提取物和 K2HPO4。进一步的高级统计方法——中心复合设计，发现筛选变量的最佳水平如下（g/L）：甘油 17.6、谷氨酸 59.6；酵母提取物 2.7；K2HPO4 2.3。在统计优化条件下，预测的聚（γ-谷氨酸）产量为 48.5 g/L，与在 7 升实验室规模发酵罐中使用 3 升工作体积进行验证实验时观察到的最大聚（γ-谷氨酸）浓度（48.7 g/L）仅相差 0.4%。
