Shandong Academy of Pharmaceutical Science, Jinan 250101, PR China.
Bioresour Technol. 2010 Jun;101(12):4733-6. doi: 10.1016/j.biortech.2010.01.128. Epub 2010 Feb 19.
In order to alleviate oxygen limitation and improve the yield of poly-gamma-glutamic acid (gamma-PGA) during fermentation, the Vitreoscilla hemoglobin gene (vgb) was integrated into the chromosome of Bacillus subtilis and expressed during gamma-PGA production. The activity of the expressed Vitreoscilla hemoglobin (VHb) was confirmed by CO-difference spectrum. Expression of VHb enhanced cell growth under high viscosity fermentation conditions 1.26-fold and increased the yield of gamma-PGA 2.07-fold. These results indicate that the expression of VHb could be advantageous in high viscosity fermentation media.
为缓解发酵过程中的供氧限制、提高聚-γ-谷氨酸（γ-PGA）产量，将血晶素基因（vgb）整合到枯草芽孢杆菌染色体中，并在 γ-PGA 生产过程中进行表达。通过 CO 差光谱证实了表达的血晶素（VHb）的活性。在高粘度发酵条件下，VHb 的表达使细胞生长提高了 1.26 倍，γ-PGA 的产率提高了 2.07 倍。这些结果表明，VHb 的表达在高粘度发酵培养基中是有利的。
