Department of Biotechnology, Autonomous Metropolitan University, Campus Iztapalapa, Av. San Rafael Atlixco 186, Col. Vicentina C.P. 09340, Iztapalapa, DF, Mexico.
Bioresour Technol. 2010 Jun;101(12):4721-4. doi: 10.1016/j.biortech.2010.01.086. Epub 2010 Feb 11.
Ferulic acid biotransformation has a number of interesting industrial uses. Ferulic acid biotransformation by the wild strain Aspergillus niger C28B25 and a diploid strain DAR2, obtained by parasexual recombination, was studied. The wild strain of A.niger C28B25 biotransforms ferulic acid to vanillic acid (VA); while the diploid strain DAR2 preferentially decarboxylates ferulic acid to 4-vinylguaiacol (4VG). The latter was identified by mass spectroscopy, (1)H and (13)C nuclear magnetic resonance spectroscopy, and quantified by HPLC. The diploid strain A.niger DAR2 and the wild strain showed a ferulic acid conversion of 64% and 36%, respectively. Molar yields show that the formation of 4VG was preferred, being as much as 4.4 times higher than the formation of VA in diploid strain cultures. Differential regulation of enzymes involved in the biotransformation of ferulic acid may explain the accumulation of 4VG by diploid DAR2. This strain produced both 4VG and VA.
阿魏酸的生物转化具有许多有趣的工业用途。研究了野生黑曲霉 C28B25 菌株和通过准性重组获得的二倍体菌株 DAR2 对阿魏酸的生物转化。野生黑曲霉 C28B25 菌株将阿魏酸转化为香草酸（VA）；而二倍体菌株 DAR2 则优先将阿魏酸脱羧基转化为 4-乙烯基愈创木酚（4VG）。通过质谱、（1）H 和（13）C 核磁共振波谱学对其进行了鉴定，并通过 HPLC 进行了定量分析。二倍体黑曲霉 DAR2 菌株和野生菌株的阿魏酸转化率分别为 64%和 36%。摩尔产率表明，4VG 的形成更为优先，在二倍体菌株的培养物中，其形成率比 VA 高 4.4 倍。阿魏酸生物转化过程中涉及的酶的差异调节可能解释了二倍体 DAR2 中 4VG 的积累。该菌株同时产生 4VG 和 VA。
