Abdelkafi Slim, Sayadi Sami, Ben Ali Gam Zouhaier, Casalot Laurence, Labat Marc
Biotechnologie Microbienne des Environnements Chauds, UR180, IRD, IFR-BAIM, Marseille Cedex, France.
FEMS Microbiol Lett. 2006 Sep;262(1):115-20. doi: 10.1111/j.1574-6968.2006.00381.x.
Halomonas elongata strain Mar (=CCUG 52759) isolated from table-olive fermentation is the first halophilic bacterium to be shown to transform ferulic acid to vanillic acid under hypersaline conditions. During growth on ferulic acid, this strain was capable of promoting the formation of a significant amount of vanillic acid and trace quantities of vanillin. The products were confirmed by high-performance liquid chromatography and gas chromatography-mass spectrometry analyses. Based on the different metabolites identified, an oxidative side chain degradation pathway of ferulic acid bioconversion to vanillic acid was suggested. Phylogenetic analysis of 16S rRNA gene revealed that this isolated strain Mar was identified as H. elongata. To increase the formation of vanillic acid, a resting cell method using H. elongata strain Mar was performed. The optimal yield of vanillic acid (86%) was obtained after a 6 h reaction using 5 mM of ferulic acid and 4 g of dry weight of cells L(-1) pregrown on ferulic acid and harvested at the end of the exponential phase.
从油橄榄发酵物中分离出的嗜盐栖热单胞菌菌株Mar（=CCUG 52759）是首个被证明在高盐条件下能将阿魏酸转化为香草酸的嗜盐细菌。在以阿魏酸为底物生长期间，该菌株能够促进大量香草酸和微量香草醛的形成。产物通过高效液相色谱和气相色谱 - 质谱分析得以确认。基于鉴定出的不同代谢产物，提出了阿魏酸生物转化为香草酸的氧化侧链降解途径。对16S rRNA基因的系统发育分析表明，分离出的菌株Mar被鉴定为嗜盐栖热单胞菌。为提高香草酸的产量，采用嗜盐栖热单胞菌菌株Mar进行了静息细胞法实验。使用5 mM阿魏酸以及4 g在阿魏酸上预生长并在指数期末收获的细胞干重每升，反应6小时后，香草酸的最佳产量（86%）得以实现。
