Departamento de Psicología, Facultad de Ciencias Humanas, Universidad Nacional de Colombia, Sede Bogotá, Colombia.
Behav Brain Res. 2010 Jun 26;210(1):67-73. doi: 10.1016/j.bbr.2010.02.011. Epub 2010 Feb 11.
The effect of intraseptal injections of lidocaine before a first or a second session in the elevated plus-maze, in a test-retest paradigm, was investigated. In addition to gross session analyses, a minute-by-minute analysis of the sessions was used to evaluate both anxiety and memory. Lidocaine injections before the test session produced increases in the frequency of entries, time spent and distance run in the open arms without affecting activity occurring in the closed arms. During the retest session, saline- and lidocaine-treated rats exhibited increased indices of anxiety and lidocaine-treated rats exhibited decreased closed-arm entries. The minute-by-minute analysis showed a faster decrease in anxiety-related behaviors during the test session by saline- than by lidocaine-treated rats and a significant decrease in closed-arm exploration by saline-treated rats, but not by lidocaine-treated ones. Lidocaine injection before the retest session produced increases in the frequency of entries, time spent and distance run in the open arms in the second session when compared with saline-treated rats. Minute-by-minute analysis showed an increase in the time spent in the open arms by lidocaine animals at the beginning of the retest session in comparison to saline animals and a significant decrease in closed-arm exploration by both groups. These results suggest that inactivation of the medial septum by lidocaine affects the expression of unconditioned and conditioned forms of anxiety in the elevated plus-maze and, in a lesser way, the acquisition and retention of spatial information.
在高架十字迷宫的测试-再测试范式中，在第一次或第二次试验前于隔核内注射利多卡因，研究其对焦虑的影响。除了总体试验分析外，还使用试验的每分钟分析来评估焦虑和记忆。在测试前注射利多卡因可增加进入开放臂的频率、时间和距离，而不影响封闭臂的活动。在再测试期间，盐水和利多卡因处理的大鼠表现出焦虑指数增加，而利多卡因处理的大鼠表现出封闭臂进入减少。每分钟分析显示，与利多卡因处理的大鼠相比，盐水处理的大鼠在测试期间焦虑相关行为的下降速度更快，且盐水处理的大鼠对封闭臂的探索减少，但利多卡因处理的大鼠没有。与盐水处理的大鼠相比，再测试前注射利多卡因可增加第二次试验中进入、停留时间和开放臂距离的频率。每分钟分析显示，与盐水处理的大鼠相比，利多卡因处理的大鼠在再测试开始时在开放臂中的停留时间增加，两组大鼠对封闭臂的探索都显著减少。这些结果表明，利多卡因对内侧隔核的失活会影响高架十字迷宫中未条件和条件性焦虑的表达，并且在较小程度上会影响空间信息的获得和保留。
