Cemagref, UR MALY, Laboratoire d'écotoxicologie, 3b quai Chauveau 69009 Lyon, France; AgroPariTech ENGREF, 19 Avenue du Maine, F 75732 Paris, France.
Chemosphere. 2010 Mar;79(1):47-53. doi: 10.1016/j.chemosphere.2010.01.030. Epub 2010 Feb 12.
The freshwater prosobranch Potamopyrgus antipodarum (Molluska, Hydrobiidea, Smith 1889) has been proposed as a suitable species to assess the impact of endocrine disrupting compounds (EDC) in aquatic ecosystems. Steroid hormone biosynthesis pathway is potentially an important target for EDC, and vertebrate-like sex steroids seem to play a functional role in the control of mollusk reproduction. To assess the response and the sensitivity of P. antipodarum to disrupters of the steroid hormone biosynthesis pathway, we have experienced the action of a specific vertebrate aromatase inhibitor, fadrozole, acting on 17beta-estradiol synthesis in two separate 28 and 42d exposures. Fadrozole had effects consistent with the expected mechanism of action. A decrease of the reproduction parameters (such as on the number of neonates and number of embryos in the brood pouch) in a dose-dependant manner was observed. The steroids levels were also impaired with the ratio 17beta-estradiol/testosterone decreased by half in exposed snails. This shift of the steroids balance was accompanied by some alteration in the gonads histology and immunohistochemistry in fadrozole-exposed snails. This study highlights the value role of P. antipodarum as a test species for assessing EDC effects in aquatic wildlife.
淡水腹足纲动物沼螺（Molluska，Hydrobiidea，Smith 1889）被提议作为评估水生生态系统中内分泌干扰化合物（EDC）影响的合适物种。甾体激素生物合成途径可能是 EDC 的一个重要靶标，而类脊椎动物性激素似乎在控制软体动物繁殖中发挥着功能作用。为了评估沼螺对甾体激素生物合成途径干扰物的反应和敏感性，我们在两个单独的 28 天和 42 天暴露实验中，体验了特定的脊椎动物芳香酶抑制剂法舒地尔对 17β-雌二醇合成的作用。法舒地尔的作用与预期的作用机制一致。观察到繁殖参数（如幼体数量和育囊中胚胎数量）以剂量依赖的方式下降。类固醇水平也受到影响，暴露的蜗牛中 17β-雌二醇/睾酮的比值降低了一半。这种类固醇平衡的转移伴随着暴露于法舒地尔的蜗牛的性腺组织学和免疫组织化学的一些改变。这项研究强调了沼螺作为评估水生野生动物中 EDC 影响的测试物种的重要作用。
