Research Center of Biomedical Engineering, Department of Biomaterials, College of Materials, Xiamen University, 422, Siming South Road, Xiamen 361005, PR China.
Biosens Bioelectron. 2011 Jan 15;26(5):1847-52. doi: 10.1016/j.bios.2010.01.027. Epub 2010 Feb 1.
An electrochemical impedance method at a fixed frequency (10Hz) is used to detect folate receptor-rich cancer cells with the interaction between folic acid immobilized on gold nanoparticles deposited on boron-doped diamond (BDD) electrode surface and its receptor over-expressed on cancer cell membrane without additional labeling. Experimental results have shown that the BDD electrode modified with folic acid can clearly denote folate receptor-rich cancer cells, such as HeLa cells, at a concentration down to 10 cells mL(-1). The high sensitivity of BDD electrode is resulted from the change of field effect and surface charge density of BDD after cancer cells attachment. The BDD electrodes can be regenerated with 0.1M citric acid/glycine/HC1 at pH 3.0 and used seven times again. Therefore, electrochemical technique with BDD electrode has been introduced for cancer cells detection and a simple method to detect folate receptor-rich cancer cells without additional labeling has been developed.
电化学阻抗法在固定频率（10Hz）下使用，通过金纳米粒子固定在硼掺杂金刚石（BDD）电极表面上的叶酸与其在细胞膜上过表达的受体之间的相互作用，无需额外标记即可检测叶酸受体丰富的癌细胞。实验结果表明，用叶酸修饰的 BDD 电极可以在浓度低至 10 个细胞 mL(-1)的情况下，明确表示叶酸受体丰富的癌细胞，如 HeLa 细胞。BDD 电极的高灵敏度源于癌细胞附着后 BDD 的场效应和表面电荷密度的变化。BDD 电极可以在 pH 3.0 的 0.1M 柠檬酸/甘氨酸/HC1 中再生，并再次使用七次。因此，电化学技术与 BDD 电极已被引入用于癌细胞检测，并开发了一种无需额外标记即可检测叶酸受体丰富的癌细胞的简单方法。
