Department of chemistry, University of Isfahan, Isfahan, 81746-73441, Iran.
Université de Lyon, Centre de Recherche en Cancérologie de Lyon, Cancer Cell Plasticity Department, UMR INSERM 1052 CNRS 5286, Centre Léon Bérard, Lyon, France.
Sci Rep. 2017 Sep 5;7(1):10513. doi: 10.1038/s41598-017-11087-2.
In the present study, a sensitive and selective signal-on method for aptamer based spectrofluorometric detection of cancer cells is introduced. AS1411, a nucleolin aptamer, is wrapped around water-soluble carbon dots and used as a probe for the detection of several types of cancer cells. Nucleolin, is overexpressed on the surface of cancer cells. Mouse breast 4T1, human breast MCF7, and human cervical HeLa cancer cells were selected as target cells, while human foreskin fibroblast cells HFFF-PI6 served as control cells. For the sensitive and selective spectrofluorimetric detection of target cancer cells in the presence of control cells, the cells were incubated in carbon dots-aptamer solutions, the cell suspensions were subsequently centrifuged and the fluorescence intensities were measured as an analytical signal. The specific targeting of cancer cells by AS1411 aptamers causes the release of carbon dots and enhances the fluorescence intensity. A calibration curve with a dynamic range between 10-4500 4T1 cells and detectability of roughly 7 cells was obtained. In addition, no significant change in the signal was detected by modifying the amount of human foreskin fibroblast control cells. Our results demonstrate similar responses to human MCF7 breast and cervical HeLa cancer cells.
在本研究中，引入了一种基于适体的灵敏和选择性信号开启方法，用于基于适体的荧光光谱法检测癌细胞。AS1411 是一种核仁素适体，它被包裹在水溶性碳点中，用作检测多种类型癌细胞的探针。核仁素在癌细胞表面过表达。选择了小鼠乳腺癌 4T1、人乳腺癌 MCF7 和人宫颈癌细胞 HeLa 作为靶细胞，而人包皮成纤维细胞 HFFF-PI6 作为对照细胞。为了在对照细胞存在的情况下对靶癌细胞进行灵敏和选择性的荧光光谱检测，将细胞在碳点-适体溶液中孵育，然后将细胞悬浮液离心，并将荧光强度作为分析信号进行测量。AS1411 适体对癌细胞的特异性靶向导致碳点的释放并增强荧光强度。在 10-4500 个 4T1 细胞之间获得了动态范围的校准曲线，检测限约为 7 个细胞。此外，通过修饰人包皮成纤维细胞对照细胞的数量，没有检测到信号的显著变化。我们的结果表明对人乳腺癌 MCF7 和宫颈癌细胞 HeLa 有相似的反应。
