成人桥本甲状腺炎与细小病毒 B19 感染相关。

Parvovirus B19 infection associated with Hashimoto's thyroiditis in adults.

机构信息

State Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital, Fourth Military Medical University, No. 17, Changle West Road, Xi'an 710032, PR China.

出版信息

J Infect. 2010 May;60(5):360-70. doi: 10.1016/j.jinf.2010.02.006. Epub 2010 Feb 12.

DOI:10.1016/j.jinf.2010.02.006

PMID:20153771

Abstract

OBJECTIVE

Parvovirus B19 is a common human pathogen, which has been linked to autoimmune diseases recently. The aim of the study is to evaluate whether B19 is involved in adult Hashimoto's thyroiditis (HT).

METHODS

Eighty-six thyroid tissues from the adult patients with a spectrum of thyroid disorders were examined for B19 DNA and capsid protein by nested PCR, in-situ hybridization and immunohistochemistry. The presence of viral DNA in HT epithelium was studied by laser-capture microdissection and sequencing of PCR products. The expressions of nuclear factor-kappaB (NF-kappaB) and interleukin-6 were investigated by immunohistochemistry.

RESULTS

B19 DNA was significantly present in HT tissues by both PCR (29/32, 90.6%) and in-situ hybridization (23/32, 71.9%, all p < 0.01) compared with normal thyroid tissue (7/16, 43.8%; 2/16, 12.5%). Laser-capture microdissection further confirmed this difference. B19 capsid protein in HT group was significantly higher than that in all the control groups (p < 0.01), and the expression of NF-kappaB and interleukin-6 in HT tissues was up-regulated. NF-kappaB was well co-localized with B19 protein in thyroid epithelia by double-labeling immunofluorescence and confocal microscopy.

CONCLUSIONS

The presence of B19 nuclear acid and viral protein was significantly common in HT tissues and it suggested a possible role of B19 in adult HT.

摘要

目的

细小病毒 B19 是一种常见的人类病原体，最近与自身免疫性疾病有关。本研究旨在评估 B19 是否参与成人桥本甲状腺炎（HT）。

方法

通过巢式 PCR、原位杂交和免疫组化检测 86 例甲状腺组织中 B19 DNA 和衣壳蛋白，对各种甲状腺疾病的成年患者进行检测。通过激光捕获微切割和 PCR 产物测序研究 HT 上皮中病毒 DNA 的存在。通过免疫组化研究核因子-κB（NF-κB）和白细胞介素-6 的表达。

结果

PCR（29/32，90.6%）和原位杂交（23/32，71.9%，均 p<0.01）均显示 HT 组织中 B19 DNA 明显存在，与正常甲状腺组织（7/16，43.8%；2/16，12.5%）相比。激光捕获微切割进一步证实了这一差异。HT 组的 B19 衣壳蛋白明显高于所有对照组（p<0.01），HT 组织中 NF-κB 和白细胞介素-6 的表达上调。双标记免疫荧光和共聚焦显微镜显示 NF-κB 在甲状腺上皮细胞中与 B19 蛋白很好地共定位。