Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, Mexico.
Fungal Genet Biol. 2010 May;47(5):446-57. doi: 10.1016/j.fgb.2010.02.004. Epub 2010 Feb 12.
The most important mechanism for fungal response to the environmental pH is the Rim or Pal pathway. Details on its operation are known through the analysis of ascomycete fungi. In this study we analyzed whether this pathway is conserved in a basidiomycete, Ustilago maydis. We could identify only five homologues of the seven known components of the pathway in the U. maydis as well as in other basidiomycete genomes. We determined that only genes encoding Rim20/PalA, Rim13/PalB and Rim23/PalC, that constitute the endosomal membrane complex, and Rim9/PalI of the complex located at the plasma membrane are conserved, but this latter lacked a detectable role in signal transduction. Mutants in this pathway showed a pleiotropic phenotype, but dimorphism and virulence were not affected. Our data reveal that the Rim/Pal pathway is conserved in basidiomycetes, but with notable differences to the ascomycete systems.
真菌响应环境 pH 的最重要机制是 Rim 或 Pal 途径。通过分析子囊菌真菌，我们了解了其运作的细节。在这项研究中，我们分析了该途径是否在担子菌尿嘧啶中保守。我们只能在 U. maydis 以及其他担子菌基因组中鉴定出该途径的七个已知成分的五个同源物。我们确定只有编码 Rim20/PalA、Rim13/PalB 和 Rim23/PalC 的基因（构成内体膜复合物）以及位于质膜上的复合物的 Rim9/PalI 是保守的，但后者在信号转导中没有可检测到的作用。该途径的突变体表现出多效表型，但二态性和毒力不受影响。我们的数据表明，Rim/Pal 途径在担子菌中是保守的，但与子囊菌系统有明显的差异。
